Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging

Cell is the structural and functional unit of organism. It serves as a key research object in various biological processes, such as growth, ontogeny, metabolism and stress. Studying the spatiotemporal distribution and functional activity of specific biological molecules in living cells is crucial fo...

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Published inFrontiers in chemistry Vol. 8; p. 598013
Main Authors Zhang, Yutong, Du, Yulin, Zhuo, Yuting, Qiu, Liping
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 11.12.2020
Subjects
aptamer
Chemistry
DNAzyme
fluorescence probe
functional nucleic acid
live-cell imaging
DNAzyme
functional nucleic acid
aptamer
fluorescence probe
live-cell imaging
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Summary:Cell is the structural and functional unit of organism. It serves as a key research object in various biological processes, such as growth, ontogeny, metabolism and stress. Studying the spatiotemporal distribution and functional activity of specific biological molecules in living cells is crucial for exploring the mechanism governing life. It also facilitates the elucidation of pathogenesis, clinical prevention and disease theranostics. In recent years, the fluorescence imaging technique has been greatly exploited for live-cell imaging. However, the development of molecular probes has lagged far behind. Functional nucleic acids (FNAs), for example, aptamer and DNAzyme, possess special chemical and/or biological functions, hence severing as promising molecular tools for cellular imaging. The current mini review focuses on the applications of FNAs in live-cell fluorescence imaging.
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Reviewed by: Hongyan Sun, City University of Hong Kong, Hong Kong; Giovanni Signore, Fondazione Pisana per la Scienza Onlus, Italy
Edited by: Jianping Yang, Donghua University, China
This article was submitted to Chemical Biology, a section of the journal Frontiers in Chemistry
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2020.598013