Is Flow Cytometry Crossmatch Analysis Using Sera with Different Dilutions Important for Pretransplant Analysis? A Case Report

• Published in: Transplantation proceedings Vol. 44; no. 6; pp. 1767 - 1769
• Main Authors: Sağıroğlu, T, Tozkır, H, Kılıçarslan-Ayna, T, Yağcı, M.A, Sezer, A, Carin, M
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.2012; Elsevier
• Subjects: Biological and medical sciences; Female; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histocompatibility; Histocompatibility Testing - methods; HLA Antigens - immunology; Humans; Isoantibodies - blood; Kidney Failure, Chronic - surgery; Kidney Transplantation - immunology; Living Donors; Male; Medical sciences; Middle Aged; Predictive Value of Tests; Reproducibility of Results; Serum - immunology; Spouses; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue, organ and graft immunology; Case study; Human; Medicine; Flow cytometry; Dilution; Treatment; Surgery; Analysis; Graft; Serum; Transplantation; Preoperative
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2012.04.019

Abstract The most effective form of treatment for chronic renal failure is kidney transplantation from a cadaver or a living donor. For a kidney transplant to be successful, tissue compatibility and a lack of donor-specific anti-human leukocyte antigen (HLA) antibodies in the circulation of the patient are vital, in addition to ABO blood group compatibility. The presence of anti-HLA antibodies is assayed before transplantation using various methods, but because organ rejections have been observed in previous studies, different techniques are required to detect anti-HLA antibodies. Today, flow cytometry crossmatching is one of the most important and effective techniques in testing for donor-specific anti-HLA antibodies (DSAs). If weakly positive serum is assayed after serial dilution, it can yield high positivity. Herein, we describe the differences between the results for diluted and undiluted weakly positive sera studied using the flow cytometry crossmatch (FCXM) technique. In a recent study, the sera of weakly FCXM-positive patients were diluted 1/50, and the FCXM test was repeated. The use of diluted serum eliminated the effect of the prozone so that the DSAs could be detected.