Linking Microstructural Integrity and Motor Cortex Excitability in Multiple Sclerosis

• Published in: Frontiers in immunology Vol. 12; p. 748357
• Main Authors: Radetz, Angela, Mladenova, Kalina, Ciolac, Dumitru, Gonzalez-Escamilla, Gabriel, Fleischer, Vinzenz, Ellwardt, Erik, Krämer, Julia, Bittner, Stefan, Meuth, Sven G, Muthuraman, Muthuraman, Groppa, Sergiu
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 12.10.2021
• Subjects: Adult; Diffusion Tensor Imaging; Disability Evaluation; Electromyography; Evoked Potentials, Motor; excitability; Female; Gray Matter - diagnostic imaging; Gray Matter - ultrastructure; Humans; Immunology; Male; Middle Aged; Models, Neurological; Motor Cortex - diagnostic imaging; Motor Cortex - physiopathology; motor threshold; multiple sclerosis; Multiple Sclerosis, Relapsing-Remitting - diagnostic imaging; Multiple Sclerosis, Relapsing-Remitting - pathology; Multiple Sclerosis, Relapsing-Remitting - physiopathology; neurite orientation dispersion and density imaging; Neurites - ultrastructure; Neuroimaging; Neuropsychological Tests; NODDI; Psychomotor Performance; tract-based spatial statistics; Transcranial Magnetic Stimulation; White Matter - diagnostic imaging; White Matter - ultrastructure; NODDI; excitability; motor threshold; tract-based spatial statistics; neurite orientation dispersion and density imaging; multiple sclerosis
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.748357

Motor skills are frequently impaired in multiple sclerosis (MS) patients following grey and white matter damage with cortical excitability abnormalities. We applied advanced diffusion imaging with 3T magnetic resonance tomography for neurite orientation dispersion and density imaging (NODDI), as well as diffusion tensor imaging (DTI) in 50 MS patients and 49 age-matched healthy controls to quantify microstructural integrity of the motor system. To assess excitability, we determined resting motor thresholds using non-invasive transcranial magnetic stimulation. As measures of cognitive-motor performance, we conducted neuropsychological assessments including the Nine-Hole Peg Test, Trail Making Test part A and B (TMT-A and TMT-B) and the Symbol Digit Modalities Test (SDMT). Patients were evaluated clinically including assessments with the Expanded Disability Status Scale. A hierarchical regression model revealed that lower neurite density index (NDI) in primary motor cortex, suggestive for axonal loss in the grey matter, predicted higher motor thresholds, i.e. reduced excitability in MS patients ( = .009, adjusted r² = 0.117). Furthermore, lower NDI was indicative of decreased cognitive-motor performance ( = .007, adjusted r² = .142 for TMT-A; = .009, adjusted r² = .129 for TMT-B; = .006, adjusted r² = .142 for SDMT). Motor WM tracts of patients were characterized by overlapping clusters of lowered NDI ( <.05, Cohen's d = 0.367) and DTI-based fractional anisotropy (FA) ( <.05, Cohen's d = 0.300), with NDI exclusively detecting a higher amount of abnormally appearing voxels. Further, orientation dispersion index of motor tracts was increased in patients compared to controls, suggesting a decreased fiber coherence ( <.05, Cohen's d = 0.232). This study establishes a link between microstructural characteristics and excitability of neural tissue, as well as cognitive-motor performance in multiple sclerosis. We further demonstrate that the NODDI parameters neurite density index and orientation dispersion index detect a larger amount of abnormally appearing voxels in patients compared to healthy controls, as opposed to the classical DTI parameter FA. Our work outlines the potential for microstructure imaging using advanced biophysical models to forecast excitability alterations in neuroinflammation.