Effect of non-esterified fatty acids on bovine granulosa cell steroidogenesis and proliferation in vitro

• Published in: Animal reproduction science Vol. 87; no. 1; pp. 33 - 44
• Main Authors: Vanholder, T., Leroy, J.L.M.R., Soom, A. Van, Opsomer, G., Maes, D., Coryn, M., Kruif, A. de
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2005
• Subjects: Animals; apoptosis; Apoptosis - drug effects; Cattle - metabolism; Cell Count; cell culture; Cell Division - drug effects; cell proliferation; Cells, Cultured; Dairy cow; dairy cows; dose response; energy balance; estradiol; Estradiol - biosynthesis; Fatty Acids, Nonesterified - pharmacology; Female; follicular development; free fatty acids; Granulosa cells; Granulosa Cells - cytology; Granulosa Cells - drug effects; Granulosa Cells - metabolism; In vitro; NEFA; oleic acid; Oleic Acid - pharmacology; palmitic acid; Palmitic Acid - pharmacology; Progesterone - biosynthesis; stearic acid; Stearic Acids - pharmacology; steroidogenesis; Steroids - biosynthesis; Granulosa cells; Dairy cow; In vitro; NEFA
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2004.09.006

In high-yielding dairy cows, the negative energy balance (NEB) during the first weeks post partum may influence dominant follicle growth and steroidogenesis. Since non-esterified fatty acid (NEFA) concentrations are elevated during NEB and are shown to be toxic for several cell types, we investigated the individual and combined effects of the three main NEFA's on granulosa cell proliferation and steroidogenesis in vitro. Granulosa cells from large follicles were cultured for two days in serum free medium in the presence of palmitic (C16:0) (PA), stearic (C18:0) (SA) and/or oleic acid (C18:1) (OA). Addition of 150, 300 or 500 μM of PA and SA inhibited cell proliferation ( P < 0.05) while OA only elicited such an effect at 500 μM ( P < 0.01). In the combination treatment (150 μM of each fatty acid), cell numbers were also reduced ( P < 0.01). These inhibitory effects on cell number are partly due to the induction of apoptosis by these NEFA's, as was demonstrated by annexin V-FITC/propidium iodide staining of the granulosa cells. Oestradiol-17β production was stimulated by all doses of PA, by 300 and 500 μM of SA and by 500 μM of OA ( P < 0.05). Combined treatment with150 μM of each fatty acid also stimulated oestradiol-17β production per 10 4 cells ( P < 0.05). We can conclude that PA, SA and to a lesser degree OA modulate granulosa cell proliferation and steroidogenesis in vitro. These effects may be involved in the occurrence of ovarian dysfunction during the postpartum period in high-yielding dairy cows.