Cloning and characterization of the gene for the metalloprotease enterotoxin of Bacteroides fragilis

The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 146; no. 2; pp. 279 - 284
Main Authors Kling, J.J, Wright, R.L, Moncrief, J.S, Wilkins, T.D
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.01.1997
Oxford University Press
Subjects
Amino Acid Sequence
Amino acids
Animal diseases
animal health
bacterial infections
Bacterial Proteins - genetics
Bacteroides fragilis
Bacteroides fragilis - enzymology
Bacteroides fragilis - genetics
Base Sequence
Cell culture
Cloning
Cloning, Molecular
Cytotoxicity
Cytotoxins
Diarrhea
DNA, Bacterial - analysis
DNA, Bacterial - isolation & purification
Enterotoxin
Enterotoxins - genetics
genbank/u67735
Gene sequencing
human health and safety
Intestine
Lipoproteins
medicine
Metalloendopeptidases - genetics
Metalloproteinase
Microbiology
Molecular Sequence Data
Nucleotides
Open reading frames
Open Reading Frames - genetics
Proteins
Sequence Analysis, DNA
Toxins
Virulence
Zinc metalloprotease
Zinc metalloprotease
Diarrhea
Enterotoxin
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Summary:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of the toxin gene (bftP). bftP is 1191 nucleotides coding for a 397 amino acid protein of 44.4 kDa. The toxin has a signal peptide of 18 amino acids that is typical of many lipoproteins followed by a 379 amino acid protoxin. The portion of the protoxin found in culture filtrates and stools begins at amino acid 212. An additional open reading frame located immediately upstream shows some sequence identity with cobra cytotoxins. If expressed, the ORF protein product could also play a role in the virulence of B. fragilis.
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ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1997.tb10205.x