Genetic architecture of the F7 gene in a Spanish population: implication for mapping complex diseases and for functional assays

• Published in: Clinical genetics Vol. 69; no. 5; pp. 420 - 428
• Main Authors: Sabater-Lleal, M, Almasy, L, Martínez-Marchán, E, Martínez-Sánchez, E, Souto, R, Blangero, J, Souto, Jc, Fontcuberta, J, Soria, JM
• Format: Journal Article
• Language: English
• Published: Oxford, UK; Malden, USA Blackwell Publishing Ltd 01.05.2006; Blackwell
• Subjects: Alleles; Amino Acid Sequence; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cardiovascular Diseases - genetics; cardiovascular risk; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; complex disease; Computational Biology; DNA Mutational Analysis; F7 variation; Factor VII - genetics; Fundamental and applied biological sciences. Psychology; FVII levels; General aspects. Genetic counseling; Genetic Predisposition to Disease; Genetics of eukaryotes. Biological and molecular evolution; Genomics; Haplotypes; Human; Humans; linkage analysis; Linkage Disequilibrium; Medical genetics; Medical sciences; Molecular and cellular biology; Molecular Sequence Data; polymorphism; Polymorphism, Single Nucleotide; Sequence Alignment; Spain; Thrombophilia - genetics; Spain; Genetic mapping; Human; Cartography; Pathogenesis; F7 variation; Variations; Cardiovascular disease; Genetic determinism; Vascular disease; Assay; Gene; linkage analysis; Analysis; Atherosclerosis; Risk factor; Genetic linkage; FVII levels; Genetics; Population; complex disease; Spanish; cardiovascular risk; Complex syndrome; Polymorphism
• ISSN: 0009-9163; 1399-0004
• DOI: 10.1111/j.1399-0004.2006.00608.x

Delineating the genetic variability of loci coding for complex diseases helps to understand the individual variation in disease susceptibility and drug response. We present the allelic architecture of the F7 gene. This gene is the major determinant of FVII plasma levels, and these plasma levels constitute an important intermediate risk factor for cardiovascular disease. As part of the Genetic Analysis of Idiopathic Thrombophila Project, we completely re‐sequenced the F7 locus (promoter, exons, introns, and 3′‐untranslated region) in 40 unrelated individuals. We found 49 polymorphisms with only two amino acid changes suggesting that regulatory non‐coding and intronic variants are responsible for the FVII variability. These results are important for mapping susceptibility alleles of complex diseases, because differences in pair‐wise linkage disequilibrium patterns between DNA variants and haplotype frequency distributions may help to detect disease‐associated alleles. In addition, we present the results of an in silico search that established genomic comparisons among different species. In conclusion, our study of the F7 DNA sequence variations is an example of a strategy for analyzing the genetic architecture of a quantitative trait locus. Furthermore, it provides a model for future analyses of genetic factors that contribute to the susceptibility of complex diseases in humans.