CDNA microarray gene expression analysis of B‐cell chronic lymphocytic leukemia proposes potential new prognostic markers involved in lymphocyte trafficking

• Published in: International journal of cancer Vol. 91; no. 4; pp. 474 - 480
• Main Authors: Stratowa, Christian, Löffler, Gerald, Lichter, Peter, Stilgenbauer, Stephan, Haberl, Peter, Schweifer, Norbert, Döhner, Hartmut, Wilgenbus, Klaus K.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 15.02.2001; Wiley-Liss
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; B‐CLL; CD18; CD18 Antigens - biosynthesis; cDNA microarrays; Cell Adhesion; DNA, Complementary - metabolism; DNA-Binding Proteins - biosynthesis; Early Growth Response Protein 1; Feasibility Studies; Female; Flow Cytometry; Hematologic and hematopoietic diseases; Humans; Immediate-Early Proteins; Immunohistochemistry; Immunophenotyping; Interleukin-1 - biosynthesis; Interleukin-8 - biosynthesis; L-Selectin - biosynthesis; Leukemia, B-Cell - diagnosis; Leukemia, B-Cell - genetics; Leukemia, B-Cell - metabolism; Leukemia, B-Cell - mortality; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphocytes - cytology; Lymphocytes - metabolism; L‐selectin; Male; Medical sciences; Middle Aged; Oligonucleotide Array Sequence Analysis; Plasmids - metabolism; Prognosis; RNA - metabolism; TCL1; Time Factors; Transcription Factors - biosynthesis; Human; Prognosis; Cytokine; Beta-Peptide chain; Cell adhesion molecule; DNA chip; Biological marker; Interleukin 1β; Malignant hemopathy; B-Lymphocyte; Gene expression; L Selectin; Cell motility; Chronic; Chronic lymphocytic leukemia; Integrin; Complementary DNA; Lymphoproliferative syndrome; Genetics; Interleukin 8
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/1097-0215(200002)9999:9999<::AID-IJC1078>3.0.CO;2-C

Human cancer is characterized by complex molecular perturbations leading to variable clinical behavior, often even in single‐disease entities. We performed a feasibility study systematically comparing large‐scale gene expression profiles with clinical features in human B‐cell chronic lymphocytic leukemia (B‐CLL). cDNA microarrays were employed to determine the expression levels of 1,024 selected genes in 54 peripheral blood lymphocyte samples obtained from patients with B‐CLL. Statistical analyses were applied to correlate the expression profiles with a number of clinical parameters including patient survival and disease staging. We were able to identify genes whose expression levels significantly correlated with patient survival and/or with clinical staging. Most of these genes code either for cell adhesion molecules (L‐selectin, integrin‐β2) or for factors inducing cell adhesion molecules (IL‐1β, IL‐8, EGR1), suggesting that prognosis of this disease may be related to a defect in lymphocyte trafficking. This report demonstrates the feasibility of a systematic integration of large‐scale gene expression profiles with clinical data as a general approach for dissecting human diseases. © 2001 Wiley‐Liss, Inc.