The zebrafish genome contains two inducible, functional cyclooxygenase-2 genes

Cyclooxygenase is a key enzyme in prostanoid biosynthesis. Mammalian species have two cyclooxygenases, constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Cox-1 and/or Cox-2 have been also identified in other vertebrates, including fish. We identified a second z...

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Published inBiochemical and biophysical research communications Vol. 352; no. 1; pp. 181 - 187
Main Authors Ishikawa, Tomo-o, Griffin, Kevin J.P., Banerjee, Utpal, Herschman, Harvey R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 05.01.2007
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cercopithecus aethiops
Conserved Sequence
COS Cells
Cyclooxygenase 2 - chemistry
Cyclooxygenase 2 - genetics
Cyclooxygenase 2 - metabolism
Danio rerio
Freshwater
Gene duplication
Genome - genetics
Isoenzymes - chemistry
Isoenzymes - genetics
Isoenzymes - metabolism
Molecular Sequence Data
Phylogeny
Prostaglandins
Sequence Alignment
Teleost genome
Zebrafish - genetics
Prostaglandins
Gene duplication
Teleost genome
Danio rerio
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Summary:Cyclooxygenase is a key enzyme in prostanoid biosynthesis. Mammalian species have two cyclooxygenases, constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Cox-1 and/or Cox-2 have been also identified in other vertebrates, including fish. We identified a second zebrafish Cox-2 gene orthologue, Cox-2b. All of the functionally important amino acids for cyclooxygenase enzymes are conserved in Cox-2b. The 3′ untranslated region of the Cox-2b message contains AU rich elements characteristic of regulation at the level of mRNA stability. Constitutive tissue expression patterns for Cox-2a and Cox-2b are distinct, but overlap. Both Cox-2a and Cox-2b expression are inducible in the kidney when fish are exposed to tetradecanoylphorbol acetate. Like Cox-2a, Cox-2b protein, expressed in COS cells is functionally active. Thus, the zebrafish genome contains two functional, inducible Cox-2 genes. Database searching demonstrates that some fish genomes contain multiple Cox-1 or Cox-2 cyclooxygenase genes, suggesting alternate duplication and retention of this gene.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.11.007