Brain energy metabolism is activated after acute and chronic administration of fenproporex in young rats

• Published in: International journal of developmental neuroscience Vol. 29; no. 8; pp. 937 - 942
• Main Authors: Rezin, Gislaine T., Jeremias, Isabela C., Ferreira, Gabriela K., Cardoso, Mariane R., Morais, Meline O.S., Gomes, Lara M., Martinello, Otaviana B., Valvassori, Samira S., Quevedo, João, Streck, Emilio L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.12.2011
• Subjects: Adolescent; Adult; Amphetamines - pharmacology; Amphetamines - therapeutic use; Animals; Brain - anatomy & histology; Brain - drug effects; Brain - metabolism; Central Nervous System Stimulants - pharmacology; Central Nervous System Stimulants - therapeutic use; Child; Citrate (si)-Synthase - metabolism; Citric Acid Cycle - drug effects; Citric Acid Cycle - physiology; Creatine Kinase - metabolism; Electron Transport - drug effects; Energy Metabolism - drug effects; Energy Metabolism - physiology; Fenproporex; Humans; Malate Dehydrogenase - metabolism; Male; Mitochondrial dysfunction; Obesity; Obesity - drug therapy; Obesity - physiopathology; Rats; Rats, Wistar; Succinate Dehydrogenase - metabolism; Fenproporex; Obesity; Mitochondrial dysfunction
• ISSN: 0736-5748; 1873-474X
• DOI: 10.1016/j.ijdevneu.2011.06.007

• Brain energy metabolism after administration of fenproporex was evaluated. • Young rats received acute or chronic administration of the drug. • The activity of some enzymes of Krebs cycle was increased by fenproporex. • Mitochondrial respiratory chain and creatine kinase were also increased by the drug. Obesity is a chronic disease of multiple etiologies, including genetic, metabolic, environmental, social, and other factors. Pharmaceutical strategies in the treatment of obesity include drugs that regulate food intake, thermo genesis, fat absorption, and fat metabolism. Fenproporex is the second most commonly consumed amphetamine-based anorectic worldwide; this drug is rapidly converted in vivo into amphetamine. Studies suggest that amphetamine induces neurotoxicity through generation of free radicals and mitochondrial apoptotic pathway by cytochrome c release, accompanied by a decrease of mitochondrial membrane potential. Mitochondria are intracellular organelles that play a crucial role in ATP production. Thus, in the present study we evaluated the activities of some enzymes of Krebs cycle, mitochondrial respiratory chain complexes and creatine kinase in the brain of young rats submitted to acute and chronic administration of fenproporex. In the acute administration, the animals received a single injection of fenproporex (6.25, 12.5 or 25 mg/kg i.p.) or tween. In the chronic administration, the animals received a single injection daily for 14 days of fenproporex (6.25, 12.5 or 25 mg/Kg i.p.). Two hours after the last injection, the rats were sacrificed by decapitation and the brain was removed for evaluation of biochemical parameters. Our results showed that the activities of citrate synthase, malate dehydrogenase and succinate dehydrogenase were increased by acute and chronic administration of fenproporex. Complexes I, II, II–III and IV and creatine kinase activities were also increased after acute and chronic administration of the drug. Our results are consistent with others reports that showed that some psychostimulant drugs increased brain energy metabolism in young rats.