Alterations in microRNA Expression during Hematopoietic Stem Cell Mobilization

• Published in: Biology (Basel, Switzerland) Vol. 10; no. 7; p. 668
• Main Authors: Nowicki, Mateusz, Szemraj, Janusz, Wierzbowska, Agnieszka, Pluta, Agnieszka, Grzybowska-Izydorczyk, Olga, Nowicka, Aleksandra, Stelmach, Piotr, Czemerska, Magdalena, Szmigielska-Kapłon, Anna
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 15.07.2021; MDPI
• Subjects: Angiogenesis; Apheresis; Apoptosis; Autografts; Bone marrow; CD34; CD34 antigen; Cell fate; Cell growth; Chemokines; Chemotherapy; Cytokines; Gene expression; Granulocytes; Growth factors; Hematology; Hematopoietic stem cells; Homeostasis; hsa-miR-146a-5p; Immunoproliferative diseases; Kinases; Leukemia; Ligands; Lymphocytes; Lymphoma; MicroRNAs; miRNA; mobilization; Multiple myeloma; Non-Hodgkin's lymphoma; Patients; Peripheral blood; Polymerase chain reaction; Stem cell transplantation; Stem cells; Tumors
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology10070668

microRNAs play an important role in the regulation of gene expression, cell fate, hematopoiesis, and may influence the efficacy of CD34+ cell mobilization. The present study examines the role of hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-34a-5p, hsa-miR-126-3p, hsa-miR-146a-5p, hsa-miR-155-5p, and hsa-miR-223-3p in the course of hematopoietic stem cell mobilization. The numbers of CD34+ cells collected in patients with hematological malignancies (39 multiple myelomas, 11 lymphomas) were determined during mobilization for an autologous hematopoietic stem cell transplantation. The miRNA level was evaluated by RT-PCR. Compared to baseline, a significant decline in hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-126-3p, hsa-miR-146a-5p, and hsa-miR-155-5p was observed on the day of the first apheresis (day A). An increase was observed only in the expression of hsa-miR-34a-5p. On day A, a negative correlation was found between hsa-miR-15a-5p and hsa-miR-146a-5p levels and the number of CD34+ cells in peripheral blood. A negative correlation was observed between hsa-miR-146a-5p and the number of collected CD34+ cells after the first apheresis. Good mobilizers, defined according to GITMO criteria, demonstrated a lower hsa-miR-146a-5p level on day A than poor mobilizers. Patients from the hsa-miR-146a-5p “low expressors” collected more CD34+ cells than “high expressors”. Our results suggest that the investigated miRNAs, especially hsa-miR-146a-5p, may influence the efficacy of HSC mobilization.