Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer

LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pat...

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Published inBiology (Basel, Switzerland) Vol. 10; no. 6; p. 500
Main Authors Lee, Jeeyong, Kwon, Junhye, Kim, DaYeon, Park, Misun, Kim, KwangSeok, Bae, InHwa, Kim, Hyunkyung, Kong, JoonSeog, Kim, Younjoo, Shin, UiSup, Kim, EunJu
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 03.06.2021
MDPI
Subjects
bio-marker
Biomarkers
Biopsy
Cancer
Cancer therapies
Cathepsin E
Chemotherapy
Colorectal cancer
DNA methylation
DNA repair
Drug dosages
Endoscopy
Epidermal growth factor
Gene expression
Immune system
Medical prognosis
Medical research
Organoids
Patients
Precision medicine
Radiation therapy
radio-responsiveness
rectal cancer
Rectum
Surgery
Tumors
United States > US
Germany
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Summary:LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pathways that participate in the radio-resistance of LARC. Thirty candidate genes encoding proteins involved in radio-responsiveness–related pathways, including the immune system, DNA repair and cell-cycle control, were identified. Interestingly, one of the candidate genes, cathepsin E (CTSE), exhibited differential methylation at the promoter region that was inversely correlated with the radio-resistance of patient-derived organoids, suggesting that methylation status could contribute to radio-responsiveness. On the basis of these results, we plan to pursue development of a gene chip for diagnosing the radio-responsiveness of LARC patients, with the hope that our efforts will ultimately improve the prognosis of LARC patients.
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These authors contributed equally.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology10060500