A novel approach to stimulate the biphenyl-degrading potential of bacterial community from PCBs-contaminated soil of e-waste recycling sites

• Published in: Bioresource technology Vol. 146; pp. 27 - 34
• Main Authors: Su, Xiaomei, Shen, Hui, Yao, Xioyan, Ding, Linxian, Yu, Chunna, Shen, Chaofeng
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.10.2013; Elsevier
• Subjects: Achromobacter - metabolism; Alcaligenes - metabolism; Applied sciences; Arthrobacter - metabolism; Bacteria; Biodegradation, Environmental; Biological and medical sciences; Biphenyl Compounds - chemistry; Biphenyl degradation; Chryseobacterium - metabolism; Communities; Conservation of Natural Resources; Culture; Degradation; Denaturing Gradient Gel Electrophoresis; Electronic Waste; Enrichment; Environmental Pollutants - metabolism; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Micrococcus luteus - metabolism; Microorganisms; Mixed-culture; Phylogeny; Pollution; Polychlorinated biphenyls; Polychlorinated Biphenyls - analysis; Polymerase Chain Reaction; Recycling; Resuscitation-promoting factor; Rhodococcus - metabolism; Soil; Soil Microbiology; Soil Pollutants - chemistry; Strain; Time Factors; Uncultured bacteria; Wastes; Polychlorinated biphenyls; Biphenyl degradation; Uncultured bacteria; Resuscitation-promoting factor; Mixed-culture; Polychlorobiphenyls; Soil pollution; Contamination; Degradation; Waste reuse; Biphenyl; Bacteria; Microbial community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.07.028

[Display omitted] •Effect of SRpf on the performance of biphenyl biodegradation was evaluated.•Biphenyl at concentration of 1500mg/L was almost completely degraded in 24h.•Six biphenyl-utilizing strains were isolated using SRpf.•A novel approach to stimulate biphenyl-degrading bacteria was proposed. SRpf, culture supernatants from Micrococcus luteus containing the resuscitation-promoting factor (Rpf), was used to enhance the biphenyl-degrading capability of potential microorganisms. The obtained results suggest that the enrichment culture produced by the addition of SRpf (enrichment culture treatment group, ECT) enhanced the biphenyl degradation efficiency, cell growth and bacterial diversity significantly. Biphenyl at concentration of 1500mg/L was almost completely degraded in 24h using SRpf at a dosage of 15% (v/v). Six strains unique to the ECT were isolated in pure cultures. This study provides a new insight into bacterial degradation of biphenyl for PCBs-bioremediation, and could be developed as a novel efficient method for obtaining highly desirable pollutant-degrading microorganisms.