Classification of the single oleosin isoform and characterization of seed oil bodies in Gymnosperms

Oleosins are hydrophobic proteins abundantly present in the oil bodies of plant seeds. Two immunologically distinct oleosins are present in seed oil bodies of diverse angiosperms, and classified as high and low M, isoforms according to their relative molecular masses in each species. Only one putati...

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Published inPlant and cell physiology Vol. 40; no. 3; pp. 326 - 334
Main Authors Wu, L.S.H. (National Chung Hsing Univ., Taichung (Taiwan)), Hong, G.H.H, Hou, R.F, Tzen, J.T.C
Format Journal Article
LanguageEnglish
Published Oxford University Press 1999
Subjects
CLASIFICACION
CLASSIFICATION
CYCAS
GIMNOSPERMAS
GINKGO BILOBA
GRAINE OLEAGINEUSE
Gymnosperm
GYMNOSPERME
GYMNOSPERMS
IMMUNOLOGICAL TECHNIQUES
Isoform
MICROSCOPIA
MICROSCOPIE
MICROSCOPY
Oil body
OILSEEDS
Oleosin
PINUS KORAIENSIS
Seed
SEMILLAS OLEAGINOSAS
TECHNIQUE IMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
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Summary:Oleosins are hydrophobic proteins abundantly present in the oil bodies of plant seeds. Two immunologically distinct oleosins are present in seed oil bodies of diverse angiosperms, and classified as high and low M, isoforms according to their relative molecular masses in each species. Only one putative oleosin was found in seed oil bodies of three representative gymnosperm species, Pinus koraiensis, Ginkgo biloba, and Cycas revoluta. The three gymnosperm oleosins were restricted to oil bodies, as detected on immuno-assaying. Immunological cross-recognition using antibodies against the three putative gymnosperm oleosins and those against the two (high and low Mr) rice oleosin isoforms suggests that the single oleosin of pine or ginkgo is immunologically related to the low Mr isoform of angiosperms, while the single cycad oleosin is immunologically distinct from both low and high Mr isoforms of angiosperms. Oil bodies were found in embryos and megagametophytes of these three species, as observed on electron microscopy. Seed oil bodies purified from these three gymnosperms maintained their integrity via electronegative repulsion and steric hindrance on the surface of the organelles. The compositions of the three essential constituents (neutral lipid, phospholipid and protein) in seed oil bodies from these three species were determined and compared with those calculated from the oil body model proposed in angiosperms. The results suggested that seed oil bodies of gymnosperms and angiosperms possess similar surface properties and structural organization
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ArticleID:40.3.326
istex:075B759D8E77EBA970D2C11A3003498BA6B4D842
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029545