Histologic Characterization of Rat Vocal Fold Scarring

This study aimed to clarify the characteristics of rat vocal fold scarring by examining the alteration of key components in the extracellular matrix: hyaluronic acid, collagen, and fibronectin. Under monitoring with a 1.9-mm-diameter telescope, unilateral vocal fold stripping was performed, and lary...

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Bibliographic Details
Published inAnnals of otology, rhinology & laryngology Vol. 114; no. 3; pp. 183 - 191
Main Authors Tateya, Tomoko, Sohn, Jin Ho, Tateya, Ichiro, Bless, Diane M.
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.03.2005
Annals Publishing Compagny
SAGE PUBLICATIONS, INC
Subjects
Animals
Biological and medical sciences
Collagen Type I - metabolism
Collagen Type III - metabolism
Fibronectins - metabolism
Hyaluronic Acid - metabolism
Immunohistochemistry
Male
Medical sciences
Otorhinolaryngology. Stomatology
Rats
Rats, Sprague-Dawley
Staining and Labeling
Vocal Cords - metabolism
Vocal Cords - pathology
collagen type I
rat model
fibronectin
vocal fold scarring
collagen type III
hyaluronic acid
Rat
Scar
Rodentia
Histology
ENT
Vocal cord
Fibronectin
Characterization
Vertebrata
Mammalia
Healing agent
Animal
Collagen
Models
Antirheumatic agent
Hyaluronic acid
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Summary:This study aimed to clarify the characteristics of rat vocal fold scarring by examining the alteration of key components in the extracellular matrix: hyaluronic acid, collagen, and fibronectin. Under monitoring with a 1.9-mm-diameter telescope, unilateral vocal fold stripping was performed, and larynges were harvested at 2, 4, 8, and 12 weeks after operation. The vocal folds were histologically analyzed with Alcian blue stain, trichrome stain, and immunofluorescence of collagen type I, collagen type III, and fibronectin. The scarred vocal folds showed less hyaluronic acid and more collagen types I and III than did the controls at all time points. Type III was stable for 12 weeks, while type I declined until 8 weeks and thereafter remained unchanged. Fibronectin increased for 4 weeks and then decreased; it was close to the control level at 8 and 12 weeks. These results suggest that the tissue remodeling process in scarred vocal folds slows down around 2 months after wounding.
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ISSN:0003-4894
1943-572X
DOI:10.1177/000348940511400303