Agar-Based Disk Diffusion Assay for Susceptibility Testing of Dermatophytes

Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is...

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Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 48; no. 10; pp. 3750 - 3752
Main Authors Nweze, E.I, Mukherjee, P.K, Ghannoum, M.A
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.10.2010
American Society for Microbiology (ASM)
Subjects
Agar
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Arthrodermataceae - drug effects
Arthrodermataceae - isolation & purification
Biological and medical sciences
Culture Media - chemistry
Diffusion
Fundamental and applied biological sciences. Psychology
Human mycoses
Humans
Infectious diseases
Medical sciences
Microbial Sensitivity Tests - methods
Microbiology
Mycology
Mycoses
Mycoses of the skin
Reproducibility of Results
Drug susceptibility test
Dermatophyte
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Summary:Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is simple, and could be used to determine the antifungal susceptibility of dermatophytes.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01357-10