Genetic Analysis of Drosophila melanogaster Polytene Chromosome Region 44D-45F: Loci Required for Viability and Fertility

A genetic screen to identify mutations in genes in the 45A region on the right arm of chromosome 2 that are involved in oogenesis in Drosophila was undertaken. Several lethal but no female sterile mutations in the region had previously been identified in screens for P-element insertion or utilizing...

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Bibliographic Details
Published inGenetics (Austin) Vol. 160; no. 4; pp. 1503 - 1510
Main Authors Mohr, Stephanie E, Boswell, Robert E
Format Journal Article
LanguageEnglish
Published United States Genetics Soc America 01.04.2002
Subjects
Animals
chromosome 2
Chromosome Mapping
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Egg Proteins - genetics
Egg Proteins - metabolism
Female
Fertility - genetics
Male
Mutation
Sequence Analysis, DNA
sine prole gene
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Summary:A genetic screen to identify mutations in genes in the 45A region on the right arm of chromosome 2 that are involved in oogenesis in Drosophila was undertaken. Several lethal but no female sterile mutations in the region had previously been identified in screens for P-element insertion or utilizing X rays or EMS as a mutagen. Here we report the identification of EMS-induced mutations in 21 essential loci in the 45D-45F region, including 13 previously unidentified loci. In addition, we isolated three mutant alleles of a newly identified locus required for fertility, sine prole. Mutations in sine prole disrupt spermatogenesis at or before individualization of spermatozoa and cause multiple defects in oogenesis, including inappropriate division of the germline cyst and arrest of oogenesis at stage 4.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/160.4.1503