Use of a D-optimal mixture design to estimate the effects of diverse chloride salts on the growth parameters of Lactobacillus pentosus

• Published in: Food microbiology Vol. 26; no. 4; pp. 396 - 403
• Main Authors: Arroyo-López, F.N., Bautista-Gallego, J., Chiesa, A., Durán-Quintana, M.C., Garrido-Fernández, A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.06.2009; Elsevier
• Subjects: Biological and medical sciences; calcium chloride; Calcium Chloride - pharmacology; chemical concentration; Chloride salts; Chlorides - pharmacology; Colony Count, Microbial; Culture Media - chemistry; Dose-Response Relationship, Drug; experimental design; Fermentation; Food industries; Food Microbiology; Food Preservation - methods; Fundamental and applied biological sciences. Psychology; ionic strength; Kinetics; Lactobacillus - drug effects; Lactobacillus - growth & development; Lactobacillus pentosus; magnesium chloride; Magnesium Chloride - pharmacology; microbial growth; Microbial Viability; Mixture design; mixtures; Models, Biological; olives; Osmolar Concentration; potassium chloride; Potassium Chloride - pharmacology; Predictive microbiology; sodium chloride; Sodium Chloride - pharmacology; Table olives; Lactobacillus pentosus; Chloride salts; Table olives; Fermentation; Mixture design; Predictive microbiology; Lactic acid bacteria; Growth; Mixture; Optimization; Olive; Salt; Chlorides; Bacteria; Lactobacillaceae; Lactobacillus
• ISSN: 0740-0020; 1095-9998
• DOI: 10.1016/j.fm.2009.01.009

The effects of NaCl, KCl, CaCl 2, and MgCl 2 and their mixtures on the ionic strength (IS) of the medium and the growth parameters of Lactobacillus pentosus were studied by means of a D-optimal mixture experimental design with constrains (total salt concentration ≤ 9.0%, wt/vol) and the generalized z-value. The IS was linearly related to the concentrations of the diverse salts and its increase, for similar concentrations of salts, followed the order MgCl 2 > CaCl 2 > NaCl > KCl. Within the experimental region, the lag phase duration ( λ) was mainly affected by NaCl and CaCl 2 and the interaction KCl with MgCl 2. The maximum specific growth rate ( μ max) decreased as NaCl (the highest effect), CaCl 2, and MgCl 2 increased (regardless of the presence or not of previous NaCl); low KCl concentrations had a stimulating effect on μ max, but its overall effect showed a similar trend to the other salts. The maximum population reached ( N max) was the least affected parameter and decreased as NaCl and CaCl 2 concentrations increased regardless of the presence of the other salts. The equations that expressed the growth parameters as a function of the diverse chloride salts, within the limits assayed, were developed and the corresponding z- and harmonic Z-values were estimated.