Characterization of Candida albicans antigenic determinants by two-dimensional polyacrylamide gel electrophoresis and enhanced chemiluminescence

• Published in: FEMS immunology and medical microbiology Vol. 23; no. 4; pp. 343 - 354
• Main Authors: Barea, Pedro Luis, Calvo, Esther, Rodriguez, José Antonio, Rementeria, Aitor, Calcedo, Roberto, Sevilla, Marı́a Jesús, Pontón, José, Hernando, Fernando Luis
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 01.04.1999; Blackwell
• Subjects: 2D SDS-PAGE; Animals; Antibodies, Fungal - immunology; Antigens; Antigens, Fungal - analysis; Biological and medical sciences; Candida albicans; Candida albicans - immunology; Chemiluminescence; Electrophoresis, Gel, Two-Dimensional - methods; Epitopes - analysis; Fundamental and applied biological sciences. Psychology; Humans; Luminescent Measurements; Mice; Microbiology; Morphology, structure, chemical composition; Mycology; Western blot; Candida albicans; Western blot; Antigens; Chemiluminescence; 2D SDS-PAGE; Human; Yeast; Antigenic determinant; Rodentia; Identification; Fungi; Antigen; Vertebrata; Mammalia; Antiserum; Mouse; Immunoblotting assay; Fungi Imperfecti; Two dimensional electrophoresis; Thallophyta
• ISSN: 0928-8244; 1574-695X
• DOI: 10.1016/S0928-8244(98)00154-0

The use of a two-dimensional polyacrylamide gel electrophoresis joined with Western blotting allowed us to investigate the reactivities of antibodies present in sera from mice and humans to antigens of Candida albicans blastoconidia. The analysis of the antibody response in the two models studied and the comparison between the antibody response in infected and non-infected individuals showed that the infection by C. albicans produces changes in the antibody response which may be of relevance in the serodiagnosis of invasive candidiasis. These changes include the induction of antibodies against new antigens, the disappearance of antibodies against a group of antigens and variations in the reactivity of antibodies directed to a different group of antigens. The technique used resolved the isoforms of several antigens including enolase. It is concluded that the antibody response in humans and mice with candidiasis is not homogeneously directed to all the isoforms of an antigen.