Long-term performance and microbial ecology of a two-stage PN–ANAMMOX process treating mature landfill leachate

• Published in: Bioresource technology Vol. 159; pp. 404 - 411
• Main Authors: Li, Huosheng, Zhou, Shaoqi, Ma, Weihao, Huang, Pengfei, Huang, Guotao, Qin, Yujie, Xu, Bin, Ouyang, Hai
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.05.2014; Elsevier
• Subjects: Air; Ammonium Compounds - metabolism; Anaerobiosis; ANAMMOX; Applied sciences; Bacteria; Bacteria - metabolism; Biodegradation, Environmental; Biological and medical sciences; Bioreactors - microbiology; Effluents; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Landfill leachate; Landfills; Nitrification; Nitrogen - isolation & purification; Nitrogen removal; Nitrosomonas; Nitrosomonas eutropha; Oxidation; Oxidation-Reduction; Partial nitritation; Phylogeny; Pollution; Reactors; Sequencing; Temperature; Wastewater treatment; Wastewaters; Water Pollutants, Chemical - isolation & purification; Water Purification - methods; Water treatment and pollution; Nitrogen removal; Partial nitritation; ANAMMOX; Landfill leachate; Wastewater treatment; Ammonium; Microbiology; Ecology; Nitrogen; Nitrosation; Long term; Sanitary landfill; Anaerobe; Bacteria; Oxidation; Waste water purification; Leachate; Denitrogenation; Maturity
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.02.054

•Endpoint pH control achieved stable partial nitritation (PN) performance.•Air flow rate and temperature had little effects on PN performance.•High rate N removal around 1kgN/m3/d was achieved by subsequent ANAMMOX unit.•Nitrosomonas sp. IWT514 (JX898090, 99%) of AOB were found in the PN reactor.•Kuenenia stuttgartiensis (CT573071, 100%) of AnAOB were found in the ANAMMOX unit. Long-term performance of a two-stage partial nitritation (PN)–anaerobic ammonium oxidation (ANAMMOX) process treating mature landfill leachate was investigated. Stable partial nitritation performance was achieved in a sequencing batch reactor (SBR) using endpoint pH control, providing an effluent with a ratio of NO2-–N/NH4+–N at 1.23±0.23. High rate nitrogen removal over 4kgN/m3/d was observed in the ANAMMOX reactor in the first three months. However, during long-term operation, the ANAMMOX reactor can only stably operate under nitrogen load of 1kgN/m3/d, with 85±1% of nitrogen removal. The ammonium oxidizing bacteria (AOB) in the PN-SBR were mainly affiliated to Nitrosomonas sp. IWT514, Nitrosomonas eutropha and Nitrosomonas eutropha, the anaerobic ammonium oxidizing bacteria (AnAOB) in the ANAMMOX reactor were mainly affiliated to Kuenenia stuttgartiensis.