CTX-M and ampC β-lactamases contributing to increased prevalence of ceftriaxone-resistant Escherichia coli in Changi General Hospital, Singapore

• Published in: Diagnostic microbiology and infectious disease Vol. 66; no. 2; pp. 210 - 213
• Main Authors: Tan, Thean Yen, Ng, Lily Siew Yong, He, Jie, Hsu, Li Yang
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2010; Elsevier
• Subjects: Aged; AmpC; Anti-Bacterial Agents - pharmacology; Bacterial Typing Techniques; Bacteriology; beta-Lactamases - biosynthesis; Biological and medical sciences; Ceftriaxone - pharmacology; Cluster Analysis; DNA Fingerprinting; Drug Resistance, Bacterial; Electrophoresis, Gel, Pulsed-Field; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Escherichia coli Proteins - biosynthesis; Female; Fundamental and applied biological sciences. Psychology; Genotype; Health-care–associated infections; Hospitals, General; Humans; Infectious Disease; Infectious diseases; Internal Medicine; Male; Medical sciences; Microbiology; Miscellaneous; Plasmid; Prevalence; Singapore; β-Lactamases, Cross-infection; Asia; South east Asia; Singapore; AmpC; Plasmid; Health-care–associated infections; β-Lactamases, Cross-infection; Cross-infection; Prevalence; Enzyme; Tropical zone; β-Lactams; Escherichia coli; Ceftriaxone; β-Lactamase; Epidemiology; Infection; Resistance; Cephalosporin derivatives; Antibiotic; Health-care-associated infections; β-Lactamases; Bacteria; Hydrolases; Antibacterial agent; Enterobacteriaceae
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2009.08.019

Abstract Data from an 800-bed hospital showed an increase in the incidence density of ceftriaxone-resistant Escherichia coli , from 13.1 to 21.7 per 100 000 inpatient days over a 4-year period. Detailed testing performed on 54 E. coli isolates showed blaCTX-M extended-spectrum β-lactamase (ESBL) genes ( n = 37) and blaCMY-like ampC genes ( n = 15). Typing data showed only limited clonal transmission in isolates with ESBL.