Retrospective analysis of the Beijing family of Mycobacterium tuberculosis in preserved lung tissues

• Published in: Journal of clinical microbiology Vol. 37; no. 2; pp. 471 - 474
• Main Authors: LISHI QIAN, VAN EMBDEN, J. D. A, VAN DER ZANDEN, A. G. M, WELTEVREDEN, E. F, DUANMU, H, DOUGLAS, J. T
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.1999
• Series: Note
• Subjects: Bacterial diseases; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Biopsy; China; Epidemiology; Fundamental and applied biological sciences. Psychology; Human bacterial diseases; Humans; Infectious diseases; Lung - microbiology; Medical sciences; Microbiology; Mycobacteriology and Aerobic Actinomycetes; Mycobacterium tuberculosis; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - isolation & purification; Nucleic Acid Hybridization; Oligodeoxyribonucleotides - analysis; Paraffin Embedding; Polymerase Chain Reaction - methods; Retrospective Studies; Tuberculosis - microbiology; Tuberculosis and atypical mycobacterial infections; Asia; China; Human; Lung disease; Typing; Respiratory disease; Lung; Spoligotype; Histological preparation; Genotype; Mycobacterial infection; Respiratory system; Retrospective; Infection; Tuberculosis; Mycobacterium tuberculosis; Mycobacteriales; Biopsy; DNA; Bacteriosis; Mycobacteriaceae; Molecular epidemiology; Bacteria; Actinomycetes
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/jcm.37.2.471-474.1999

Direct repeat spoligotyping of 85 paraffin-embedded lung biopsies was used to investigated the occurrence around Beijing of the Beijing family of Mycobacterium tuberculosis. Samples ranged in time from 1956 to 1990. Hybridization patterns were found with 49 (58%) samples, and 45 (92%) produced typical Beijing family patterns extending over the 34-year period.