Determination of oxidative stress and cellular inflammation in patients with diabetic nephropathy and non-diabetic nephropathy being administered hemodialysis treatment due to chronic renal failure

• Published in: Renal failure Vol. 36; no. 5; pp. 767 - 773
• Main Authors: Avci, Emre, Çakir, Erdinc, Cevher, Sule Coskun, Yaman, Halil, Agilli, Mehmet, Bilgi, Cumhur
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare USA, Inc 01.06.2014; Taylor & Francis
• Subjects: Aged; Arginine - analogs & derivatives; Arginine - blood; Biomarkers - blood; C-Reactive Protein - metabolism; Case-Control Studies; Cellular inflammation; chronic renal failure; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - blood; Diabetic Nephropathies - blood; Diabetic Nephropathies - complications; diabetic nephropathy; Female; hemodialysis; Humans; Inflammation - blood; Inflammation - etiology; Interleukin-6 - blood; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - complications; Male; Malondialdehyde - blood; Middle Aged; Neopterin - blood; Oxidative Stress; Tumor Necrosis Factor-alpha - blood; hemodialysis; Cellular inflammation; chronic renal failure; diabetic nephropathy; oxidative stress
• ISSN: 0886-022X; 1525-6049
• DOI: 10.3109/0886022X.2014.890841

Abstract Objectives: We aimed to evaluate oxidative stress [8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA)] endothelial damage [asymmetric dimethylarginine (ADMA)] and markers of cellular inflammation [interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), neopterin (NP) and high-sensitivity C-reactive protein (hsCRP)] in patients with diabetic nephropathy (DN) and non-diabetic nephropathy who were being administered hemodialysis treatment because of chronic renal failure. Methods: In determining 8-OHdG, IL-6 and TNF-α levels, Enzyme-Linked Immuno-Sorbent Assay method was used. Serum MDA, ADMA and NP levels were determined by using high performance liquid chromatography (HPLC). And hs-CRP values were measured with nephelometric method. Results: Serum 8-OHdG and MDA levels were found statistically to have increased when compared with those of the control group in patients groups after dialysis. However, serum ADMA and neopterin levels were observed statistically to have decreased when compared with those of the control group in patients groups after dialysis. But, decreases on ADMA and neopterin levels are still much higher than those of control. IL-6 and TNF-α levels were found to have increased when compared with those of control group in patients groups before dialysis. Conclusion: The oxidative stress in patients with DN, who were being treated with hemodialysis due to chronic renal failure, was higher than that of non-DN patients who were being treated with hemodialysis. In contrast with this, inflammation occurring in non-DN patients was found to have been higher than that of in patients with DN.