A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2

Two polyclonal rabbit antibodies to epithelial membrane antigen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using an indirect immunoperoxidase technique. Sections from 25 tissues (17 malignant and 8 benign), were exa...

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Bibliographic Details
Published inBritish journal of cancer Vol. 52; no. 3; pp. 355 - 361
Main Authors HEYDERMAN, E, STRUDLEY, I, POWELL, G, RICHARDSON, T. C, CORDELL, J. L, MASON, D. Y
Format Journal Article
LanguageEnglish
Published Basingstoke Nature Publishing Group 01.09.1985
Subjects
Antibodies - immunology
Antibodies, Monoclonal - immunology
Antigens, Neoplasm - analysis
Biological and medical sciences
Breast - immunology
Breast Neoplasms - immunology
Colonic Neoplasms - immunology
Epithelium - immunology
Female
Humans
Immunoenzyme Techniques
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Membrane Proteins - immunology
Miscellaneous. Technology
Mucin-1
Neoplasms - diagnosis
Neoplasms - immunology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Human
Immunohistochemistry
Pathology
Plasma membrane
Tumor
Monoclonal antibody
Epithelium
Comparative study
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Summary:Two polyclonal rabbit antibodies to epithelial membrane antigen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using an indirect immunoperoxidase technique. Sections from 25 tissues (17 malignant and 8 benign), were examined. The distribution of staining with each of these reagents was similar, but the polyclonal antibodies produced stronger staining in colorectal carcinomas and lactating breast, whereas staining with the monoclonal antibodies was stronger in non-neoplastic pleural mesothelium and in pulmonary alveolar cells. When the two monoclonals were mixed there was no increase in staining intensity. E29 gave a "cleaner" result than HMFG-2, with better discrimination between cells and stroma, and is highly suitable for routine diagnostic histopathology.
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ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1985.201