Formation of di-isodityrosine and loss of isodityrosine in the cell walls of tomato cell-suspension cultures treated with fungal elicitors or H2O2

• Published in: Plant physiology (Bethesda) Vol. 115; no. 1; pp. 87 - 92
• Main Authors: Brady, J.D, Fry, S.C
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.09.1997
• Subjects: AGENT PATHOGENE; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; AUREOBASIDIUM PULLULANS; BIOCHEMICAL PATHWAYS; Biological and medical sciences; CELL CULTURE; CELL WALLS; CHEMICAL REACTIONS; CHEMICAL STRUCTURE; CULTIVO DE CELULAS; CULTURE DE CELLULE; DERIVATIVES; DISEASE RESISTANCE; ENFERMEDADES FUNGOSAS; ESTRUCTURA QUIMICA; Fundamental and applied biological sciences. Psychology; FUNGAL DISEASES; Fungal plant pathogens; GLICOPROTEINAS; GLYCOPROTEINE; GLYCOPROTEINS; HIBRIDOS; HIDROXIPROLINA; HYBRIDE; HYBRIDS; HYDROGEN PEROXIDE; HYDROXYPROLINE; INDUCTION; INFECTIVITY; LYCOPERSICON ESCULENTUM; LYCOPERSICON PERUVIANUM; MALADIE FONGIQUE; Metabolism; Metabolism. Physicochemical requirements; METABOLITE; METABOLITES; METABOLITOS; ORGANISMOS PATOGENOS; OXIDACION; OXIDATION; OXYDATION; PARED CELULAR; PAROI CELLULAIRE; PATHOGENICITY; PATHOGENS; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; PATOGENICIDAD; PEROXIDO DE HIDROGENO; PEROXYDE D'HYDROGENE; Phytopathology. Animal pests. Plant and forest protection; PHYTOPHTHORA MEGASPERMA; Plant physiology and development; POUVOIR PATHOGENE; PYTHIUM APHANIDERMATUM; QUANTITATIVE ANALYSIS; REACCIONES QUIMICAS; REACTION CHIMIQUE; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; STRUCTURE CHIMIQUE; TIROSINA; TYROSINE; VIA BIOQUIMICA DEL METABOLISMO; VOIE BIOCHIMIQUE DU METABOLISME; Cell culture; Plant pathogen; Hydroxyproline; Crosslinking; Glycoproteins; Elicitor; Hydrogen Peroxides; Cell wall; Fungi; Dicotyledones; Angiospermae; Defense mechanism; Spermatophyta; Solanaceae; Pretreatment; Thallophyta; Quantitative analysis
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.115.1.87

About 84% of the hydroxyproline residues in a cell culture of tomato (Lycopersicon esculentum X Lycopersicon peruvianum) were present in phenol-inextractable (i.e. covalently wall-bound) material. Treatment of the cells with any of three fungal elicitors (wall fragments from Phytophthora megasperma and Pythium aphanidermatum and xylanase from Aureobasidium pullulans) or with 1 mM H2O2 had little effect on the quantity of phenol-inextractable hydroxyproline per milligram of freeze-dried cells. However, each treatment induced a decrease in the content of phenol-inextractable isodityrosine (Idt) residues. Each treatment, except with the P. megasperma fragments, also induced an increase in phenol-inextractable di- (Di-Idt). The increase in Di-Idt partly accounted for the loss of Idt. We conclude that the elicitors and H2O2 acted to reinforce the existing cross-linking of cell wall (glyco) proteins by evoking oxidative coupling reactions to convert Idt to Di-Idt plus unidentified products. The promotion of cross-linking by elicitor treatment is proposed to be a defensive response that restricts the penetration of pathogens