Blockade of the epidermal growth factor receptor decreases intimal hyperplasia in balloon-injured rat carotid artery

• Published in: Journal of vascular surgery Vol. 37; no. 3; pp. 644 - 649
• Main Authors: Chan, Allen K., Kalmes, Andreas, Hawkins, Suzanne, Daum, Günter, Clowes, Alexander W.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.2003; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - administration & dosage; Biological and medical sciences; Carotid Artery Injuries - pathology; Carotid Artery, Common - pathology; Catheterization; Cell Division; Diseases of the cardiovascular system; Hyperplasia; Immunoglobulin G - pharmacology; Male; Medical sciences; Muscle, Smooth, Vascular - pathology; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Sprague-Dawley; Receptor, Epidermal Growth Factor - immunology; Receptor, Epidermal Growth Factor - physiology; Tunica Intima - pathology; Cell proliferation; Rat; Pathogenesis; Hyperplasia; Rodentia; Instrumentation therapy; Cuffed tube; Smooth muscle; Instrumental dilatation; Restenosis; Vertebrata; Mammalia; Epidermal growth factor; Treatment; Follow up study; Animal; Carotid; Intima; Complication
• ISSN: 0741-5214; 1097-6809
• DOI: 10.1067/mva.2003.92

Hypothesis: Arterial intimal hyperplasia is induced by injury and is frequently the cause of luminal narrowing after vascular reconstruction. Smooth muscle cells (SMC) respond to injury by proliferating and migrating into the intima. This process is regulated by thrombin, endothelin, and angiotensin II, all ligands of G protein-coupled receptors. Signal transduction from these receptors in cultured cells depends in part on transactivation of epidermal growth factor receptor (EGFR). We hypothesize that EGFR has a substantial role in activation of SMC in vivo and development of intimal hyperplasia. Methods: Intimal hyperplasia was induced in rat carotid arteries by passage of a balloon catheter. Animals were given a monoclonal blocking antibody to rat EGFR, matched mouse immunoglobulin G (IgG) control antibody, or saline solution. Results: Blocking EGFR antibody inhibited medial SMC proliferation, as determined by 5-bromo-2′-deoxyuridine labeling at 2 days (IgG control, 8.0% ± 2.0%; anti-EGFR, 3.2% ± 0.8%) and intimal hyperplasia at 14 days (intimal area: IgG control, 0.07 ± 0.01 mm2; anti-EGFR, 0.04 ± 0.01 mm2). Conclusion: Activation of EGFR is important for early induction of SMC proliferation and subsequent intimal thickening. (J Vasc Surg 2003;37:644-9.)