Highly efficient extraction and lipase-catalyzed transesterification of triglycerides from Chlorella sp. KR-1 for production of biodiesel

• Published in: Bioresource technology Vol. 147; pp. 240 - 245
• Main Authors: Lee, Ok Kyung, Kim, Young Hyun, Na, Jeong-Geol, Oh, You-Kwan, Lee, Eun Yeol
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2013; Elsevier
• Subjects: Biocatalysis; Biofuel production; Biofuels; Biological and medical sciences; Biotechnology; Bulk molding compounds; Chlorella; Chlorella - metabolism; Chlorella sp. KR-1; Chromatography, Gas; Conversion; Dimethyl carbonate; Energy; Esterification; Extraction; Fatty acid methyl esters; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Lipase - metabolism; Lipids; Methyl alcohol; Microalgae; Novozyme 435; Transesterification; Triglycerides; Triglycerides - isolation & purification; Triglycerides - metabolism; Microalgae; Chlorella sp. KR-1; Dimethyl carbonate; Fatty acid methyl esters; Novozyme 435; Chlorella; Enzyme; Algae; Enzymatic catalysis; Transesterification; Chlorophyceae; Triglyceride; Biodiesel; Chlorophyta; Alga; Carbonates; Microorganism; Fatty acid methyl ester; Lipolytic enzyme
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.08.037

•Lipid of Chlorella sp. KR-1 was extracted using dimethyl carbonate (DMC) and methanol.•DMC was also used as acyl acceptor and reaction solvent.•FAMEs with a yield of 293mg/g biomass were obtained using Novozyme 435 in DMC.•Novozyme 435 was re-used for ten times with activity loss below 10% of initial value. We developed a method for the highly efficient lipid extraction and lipase-catalyzed transesterification of triglyceride from Chlorella sp. KR-1 using dimethyl carbonate (DMC). Almost all of the total lipids, approximately 38.9% (w/w) of microalgae dry weight, were extracted from the dried microalgae biomass using a DMC and methanol mixture (7:3 (v/v)). The extracted triglycerides were transesterified into fatty acid methyl esters (FAMEs) using Novozyme 435 as the biocatalyst in DMC. Herein, DMC was used as the reaction medium and acyl acceptor. The reaction conditions were optimized and the FAMEs yield was 293.82mg FAMEs/g biomass in 6h of reaction time at 60°C in the presence of 0.2% (v/v) water. Novozyme 435 was reused more than ten times while maintaining relative FAMEs conversion that was greater than 90% of the initial FAMEs conversion.