Measuring the activity of heterotrophic microorganism in membrane bioreactor for drinking water treatment

• Published in: Bioresource technology Vol. 130; pp. 136 - 143
• Main Authors: Han, Zheng-Shuang, Tian, Jia-Yu, Liang, Heng, Ma, Jun, Yu, Hua-Rong, Li, Kai, Ding, An, Li, Gui-Bai
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2013; Elsevier
• Subjects: Biological and medical sciences; Biomass; Biomass respiration potential; Bioreactors; Bioreactors - microbiology; Biotechnology; Consistency; Dehydrogenase activity; Drinking water; Feasibility Studies; Fundamental and applied biological sciences. Psychology; Heterotrophic microorganism activity; Heterotrophic Processes; Membrane adsorption bioreactor; Membrane bioreactor; Membranes; Membranes, Artificial; Methods. Procedures. Technologies; Microorganisms; Optimization; Oxidoreductases - metabolism; Oxygen - metabolism; Sewage; Sludge; Temperature; Tetrazolium Salts - metabolism; Various methods and equipments; Water Purification; MAOCC; BRP; Membrane bioreactor; Heterotrophic microorganism activity; TTC; Biomass respiration potential; MABR; BDOC; DO; HRT; SRT; AOC; Membrane adsorption bioreactor; BOD; DOC; MBR; SS; BOM; DOM; BAC; Dehydrogenase activity; OUR; THOD; TF; PAC; TMP; VSS; DHA; ATP; Drinking water treatment; Enzyme; Heterotrophy; Biomass; Dehydrogenase; Membrane reactor; Bioreactor; Enzymatic activity; Adsorption; Oxidoreductases; Microorganism; Respiration
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.11.151

► BRP & TTC–DHA tests were modified to measure biomass activity in drinking water MBR. ► The optimal parameters for TTC–DHA test were obtained. ► Both BRP and TTC–DHA tests were feasible for assessing biological performance in MBR. ► Correlation between the BRP and TTC–DHA was established. ► BRP test was feasible for assessing biological performance in MABR. In order to quantify the activity of heterotrophic microorganism in membrane bioreactor (MBR) for drinking water treatment, biomass respiration potential (BRP) test and 2,3,5-triphenyl tetrazolium chloride–dehydrogenase activity (TTC–DHA) test were introduced and modified. A sludge concentration ratio of 5:1, incubation time of 2h, an incubation temperature that was close to the real operational temperature, and using a mixture of main AOC components as the substrate were adopted as the optimum parameters for determination of DHA in drinking water MBR. A remarkable consistency among BDOC removal, BRP and DHA for assessing biological performance in different MBRs was achieved. Moreover, a significant correlation between the BRP and DHA results of different MBRs was obtained. However, the TTC–DHA test was expected to be inaccurate for quantifying the biomass activity in membrane adsorption bioreactor (MABR), while the BRP test turned out to be still feasible in that case.