A highly prevalent filamentous algal endophyte in natural populations of the sugar kelp Saccharina latissima is not detected during cultivation in Northern Brittany

• Published in: Aquatic living resources (Montrouge) Vol. 32; no. 21; pp. 21 - 26
• Main Authors: Bernard, Miriam, Rousvoal, Sylvie, Collet, Nadia, Le Goff, Tristan, Jacquemin, Bertrand, Peters, Akira, Potin, Philippe, Leblanc, Catherine, Carnegie, Ryan B
• Format: Journal Article
• Language: English
• Published: Les Ulis EDP Sciences 2019
• Subjects: Algae; Chemical analysis; Collectors; Controlled conditions; Cultivation; Deoxyribonucleic acid; Deployment; DNA; Endophytes; Farms; Fertility; Filaments; Food contamination; Genetic testing; Infections; Kelp; Laminarionema elsbetiae; Life Sciences; Marine; Natural populations; PCR; Phytopathology and phytopharmacy; Polyethylene; Populations; quantitative PCR; Renewable energy; Ropes; Saccharides; Saccharina latissima; Seawater; seaweed aquaculture; Seaweed culture; Seaweeds; Seeding; Spores; Sporophytes; Vegetal Biology; Water analysis; ANE, France, Brittany; United States > US; Europe; Germany; Endophytes; Saccharina latissima; seaweed aquaculture; Laminarionema elsbetiae; quantitative PCR
• ISSN: 1765-2952; 0990-7440; 1765-2952
• DOI: 10.1051/alr/2019019

The sugar kelp Saccharina latissima is cultivated in Europe for food, feed and ultimately the production of chemical commodities and bioenergy. Being cultivated in the open sea, S. latissima is exposed to potentially harmful organisms, such as Laminarionema elsbetiae, a filamentous brown algal endophyte with a very high prevalence in wild populations of European S. latissima. As it was shown previously that S. latissima sporophytes get infected by L. elsbetiae very early in their life, seeding the spores on collectors and keeping them under controlled conditions during the critical time of a possible infection with filamentous endophytes could be advantageous over direct seeding techniques, where the ropes are deployed within days after seeding. We used a qPCR-assay to assess the prevalence of the endophyte L. elsbetiae in S. latissima cultivated during winter in Northern Brittany, comparing individuals from direct-seeded ropes and collector-seeded lines that were kept in laboratory conditions for different time spans. No DNA of the endophyte was detected in the samples, suggesting that either the kelps were not infected or the amount of endophytic filaments were below the detection rate of the qPCR assay. Furthermore, L. elsbetiae could not be detected in the seawater surrounding the kelp farm, indicating that L. elsbetiae is not fertile or disperses at a very small scale in Northern Brittany during the deployment time of young kelps. Our results suggest that infections of cultivated S. latissima with the endophyte L. elsbetiae might be a minor problem in kelp farms in Northern Brittany if the seeding production is kept under controlled conditions without external contamination.