Histopathological changes and antioxidant response in brain and kidney of common carp exposed to atrazine and chlorpyrifos

• Published in: Chemosphere (Oxford) Vol. 88; no. 4; pp. 377 - 383
• Main Authors: Xing, Houjuan, Li, Shu, Wang, Zhilei, Gao, Xuejiao, Xu, Shiwen, Wang, Xiaolong
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2012; Elsevier
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Animals; antioxidants; Antioxidants - metabolism; Applied ecology; Atrazine; Atrazine - toxicity; Biological and medical sciences; brain; Brain - cytology; Brain - drug effects; Brain - enzymology; Brain - metabolism; Carps - metabolism; catalase; Catalase - metabolism; Chlorpyrifos; Chlorpyrifos - toxicity; Cyprinus carpio; Cyprinus carpio L; Ecotoxicology, biological effects of pollution; fish; Fresh water ecosystems; Fundamental and applied biological sciences. Psychology; General aspects; glutathione peroxidase; Glutathione Peroxidase - metabolism; histopathology; Histopathology changes; Kidney - cytology; Kidney - drug effects; Kidney - enzymology; Kidney - metabolism; kidneys; malondialdehyde; Malondialdehyde - metabolism; Oxidative stress; stress response; subchronic exposure; superoxide dismutase; Superoxide Dismutase - metabolism; Synecology; Water Pollutants, Chemical - toxicity; Chlorpyrifos; Oxidative stress; Cyprinus carpio L; Histopathology changes; Atrazine; Brain; Antioxidant; Kidney; Freshwater environment; Vertebrata; Histopathology; Pisces; Cyprinus carpio
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2012.02.049

► Oxidative stress and histopathology were investigated by ATR, CPF and their mixture. ► Our aim was to evaluate the sub-chronic effects of ATR, CPF and their mixture on common carp. ► This is the first report of oxidative stress and histopathology to study the subchronic effects of ATR and CPF on carp. We investigated oxidative stress response and histopathological changes in the brain and kidney of the common carp after a 40-d exposure to CPF and ATR, alone or in combination, and a 20-d recovery. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) content were measured using standard assays. Our results indicated that exposure to ATR, CPF or a combination of the two for 40d induced significant changes in antioxidant enzyme (SOD, CAT and GSH-Px) activities and MDA content in the brain and kidney of the common carp. Pathological changes included tissue damage that was more severe with increased of exposure dose. To our knowledge, this is the first report to study oxidative stress and histopathological effects caused by subchronic exposure to ATR, CPF and ATR/CPF combination on common carp. The information presented in this study may be helpful to understanding the mechanisms of ATR-, CPF- and ATR/CPF combination-induced oxidative stress in fish.