Development of an orange juice in-house reference material and its application to guarantee the quality of vitamin C determination in fruits, juices and fruit pulps

• Published in: Food chemistry Vol. 154; pp. 71 - 77
• Main Authors: Valente, A., Sanches-Silva, A., Albuquerque, T.G., Costa, H.S.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2014; Elsevier
• Subjects: Ascorbic acid; Ascorbic Acid - analysis; Beverages - analysis; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Citrus sinensis - chemistry; Food toxicology; Fruit - chemistry; Medical sciences; Quality Control; Reference material; Reference Standards; Toxicology; Validation; Vitamin C; Waste Products - analysis; Validation; Reference material; Ascorbic acid; Vitamin C; Quality control; Vitamin; Housing; Fruit juice; Pulp; Micronutrient; Assay; Orange juice; Quality; Development; Food; Quantitative analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.12.053

•A new reference material for quality control in vitamin C analysis was developed.•The newly developed stabilization solution minimizes the degree of ascorbic acid loss.•Reference material exhibits suitable homogeneity.•Short and long-term stability were proven for materials stored frozen.•This reference material is suitable for its intended use. Reference materials are useful for the quality control of analytical procedures and to evaluate the performance of laboratories. There are few and expensive certified reference materials commercially available for vitamin C or ascorbic acid analysis in food matrices. In this study, the preparation and the suitability assessment of an orange juice in-house reference material (RM) for vitamin C analysis in fruits, juices and in fruit pulps is described. This RM was used for the development and full validation of an HPLC method. The results showed excellent linearity (r2=0.9995), good accuracy (96.6–97.3%) and precision, as relative standard deviation, ranged from 0.70% to 3.67%. The in-house RM was homogenous and stable at storage conditions (−80°C) during 12months. According to our results, this in-house RM is an excellent tool to use in quality control and method verification purposes for vitamin C analysis of fruits, juices and fruit pulps matrices. Furthermore, a stabilization solution with perchloric and metaphosphoric acids was developed which prevents degradation of ascorbic acid for a period of 12months at −80°C.