Archaeal community dynamics and abiotic characteristics in a mesophilic anaerobic co-digestion process treating fruit and vegetable processing waste sludge with chopped fresh artichoke waste

• Published in: Bioresource technology Vol. 136; pp. 1 - 7
• Main Authors: Ros, M., Franke-Whittle, I.H., Morales, A.B., Insam, H., Ayuso, M., Pascual, J.A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.05.2013; Elsevier
• Subjects: Ammonia - analysis; Anaerobic digestion; Anaerobiosis; ANAEROCHIP; Applied sciences; Archaea - genetics; Archaea - metabolism; Artichokes; Biodegradation, Environmental; Biofuels; Biogas; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Bioreactors; Biotechnology; Co-digestion; Communities; Cynara scolymus - chemistry; Environment and pollution; Exact sciences and technology; Fatty Acids, Volatile - analysis; Fruit - chemistry; Fruits; Fundamental and applied biological sciences. Psychology; Gene Dosage - genetics; Industrial applications and implications. Economical aspects; Methane; Methanosaeta; Methanosarcina; Methods. Procedures. Technologies; Oligonucleotide Array Sequence Analysis; Pollution; Principal Component Analysis; Real time PCR; Refuse Disposal - methods; RNA, Ribosomal, 16S - genetics; Sequential batch reactor; Sewage - microbiology; Sludge; Various methods and equipments; Vegetables; Vegetables - chemistry; Volatilization; Waste Products - analysis; Wastes; ANAEROCHIP; Real time PCR; Sequential batch reactor; Anaerobic digestion; Co-digestion; Dynamic characteristic; Vegetables; Fruit; Environmental factor; Compositae; Real time; Abiotic factor; Polymerase chain reaction; Vegetable crop; Sequencing batch reactor; Cynara cardunculus; Dicotyledones; Angiospermae; Artichoke; Mesophily; Spermatophyta; Wastes; Community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.02.058

► Co-digestion of fruit and vegetable waste sludge with artichoke waste enhanced biogas production. ► Methanosaeta and Methanosarcina dominanted the methanogenic community. ► Methanosarcina numbers increased upon co-digestion with chopped fresh artichoke waste. ► Methanosaeta numbers decreased upon co-digestion with chopped fresh artichoke waste. This study evaluated the feasibility of obtaining methane in anaerobic digestion (AD) from the waste products generated by the processing of fruit and vegetables. During the first phase (0–55d) of the AD using sludge from fruit and vegetable processing, an average value of 244±88Lkg−1dry matterd−1of biogas production was obtained, and methane content reached 65% of the biogas. Co-digestion with chopped fresh artichoke wastes in a second phase (55–71d) enhanced biogas production, and resulted in an average value of 354±68Lkg−1dry matterd−1, with higher methane content (more than 70%). The archaeal community involved in methane production was studied using the ANAEROCHIP microarray and real-time PCR. Results indicated that species of Methanosaeta and Methanosarcina were important during the AD process. Methanosarcina numbers increased after the addition of chopped fresh artichoke, while Methanosaeta numbers decreased.