A Warp-Knitted Light-Emitting Fabric-Based Device for In Vitro Photodynamic Therapy: Description, Characterization, and Application on Human Cancer Cell Lines

Photodynamic therapy (PDT) appears to be a promising strategy in biomedical applications. However, the complexity of its parameters prevents wide acceptance. This work presents and characterizes a novel optical device based on knitted light-emitting fabrics and dedicated to in vitro PDT involving lo...

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Published inCancers Vol. 13; no. 16; p. 4109
Main Authors Thécua, Elise, Ziane, Laurine, Grolez, Guillaume Paul, Fagart, Alexandre, Kumar, Abhishek, Leroux, Bertrand, Baert, Gregory, Deleporte, Pascal, Vermandel, Maximilien, Vignion-Dewalle, Anne-Sophie, Delhem, Nadira, Mordon, Serge
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 15.08.2021
MDPI
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Summary:Photodynamic therapy (PDT) appears to be a promising strategy in biomedical applications. However, the complexity of its parameters prevents wide acceptance. This work presents and characterizes a novel optical device based on knitted light-emitting fabrics and dedicated to in vitro PDT involving low irradiance over a long illumination period. Technical characterization of this device, called CELL-LEF, is performed. A cytotoxic study of 5-ALA-mediated PDT on human cancer cell lines is provided as a proof of concept. The target of delivering an irradiance of 1 mW/cm2 over 750 cm2 is achieved (mean: 0.99 mW/cm2; standard deviation: 0.13 mW/cm2). The device can maintain a stable temperature with the mean thermal distribution of 35.1 °C (min: 30.7 °C; max: 38.4 °C). In vitro outcomes show that 5-ALA PDT using CELL-LEF consistently and effectively induced a decrease in tumor cell viability: Almost all the HepG2 cells died after 80 min of illumination, while less than 60% of U87 cell viability remained. CELL-LEF is suitable for in vitro PDT involving low irradiance over a long illumination period.
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Elise Thécua and Laurine Ziane contributed equally to this work.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers13164109