Addition of feruloyl esterase and xylanase produced on-site improves sugarcane bagasse hydrolysis

• Published in: Bioresource technology Vol. 170; pp. 316 - 324
• Main Authors: Braga, Cleiton Márcio Pinto, Delabona, Priscila da Silva, Lima, Deise Juliana da Silva, Paixão, Douglas Antônio Alvaredo, Pradella, José Geraldo da Cruz, Farinas, Cristiane Sanchez
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.10.2014; Elsevier
• Subjects: Accessory enzymes; Biofuels; Biological and medical sciences; Biomass; Bioreactors; Biotechnology; Carboxylic Ester Hydrolases - metabolism; Carboxylic Ester Hydrolases - pharmacology; Cellulose - metabolism; Endo-1,4-beta Xylanases - metabolism; Endo-1,4-beta Xylanases - pharmacology; Enzyme engineering; Eurotiales - enzymology; Feruloyl esterase; Food industries; Fundamental and applied biological sciences. Psychology; Hydrolysis - drug effects; Methods. Procedures. Technologies; On-site enzyme production; Production of selected enzymes; Saccharum - chemistry; Sugarcane bagasse; Use and upgrading of agricultural and food by-products. Biotechnology; Xylanase; On-site enzyme production; Accessory enzymes; Feruloyl esterase; Xylanase; Sugarcane bagasse; Hydrolysis; Sugar cane(by product); Enzyme; Bagasse; In situ; Enzyme production; Xylanolytic enzyme
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.07.115

•Accessory enzymes produced on-site can improve biomass degradation.•Feruloyl esterase and xylanase improved sugarcane bagasse hydrolysis.•Sugarcane bagasse hydrolysis increased 36% with supplementation. Accessory enzymes that assist biomass degradation could be used to improve the recovery of fermentable sugar for use in biorefineries. In this study, different fungal strains isolated from the Amazon rainforest were evaluated in terms of their ability to produce feruloyl esterase (FAE) and xylanase enzymes, and an assessment was made of the contributions of the enzymes in the hydrolysis of pretreated sugarcane bagasse. In the selection step, screening using plate assays was followed by shake flask submerged cultivations. After carbon source selection and cultivation in a stirred-tank bioreactor, Aspergillusoryzae P21C3 proved to be a promising strain for production of the enzymes. Supplementation of a commercial enzyme preparation with 30% (v/v) crude enzymatic complex from A. oryzae P21C3 increased the conversion of cellulose derived from pretreated sugarcane bagasse by 36%. Supplementation with FAE and xylanase enzymes produced on-site can therefore be used to improve the hydrolysis of sugarcane bagasse.