Development of Leishmania (Leishmania) infantum chagasi in Its Natural Sandfly Vector Lutzomyia longipalpis

We analyzed the development of Leishmania (Leishmania) infantum chagasi in its natural sandfly vector Lutzomyia longipalpis. In addition, we compared sandfly infections initiated with axenic amastigotes or promastigotes. Our data showed no important difference between Lu. longipalpis infection rates...

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Bibliographic Details
Published inThe American journal of tropical medicine and hygiene Vol. 86; no. 4; pp. 606 - 612
Main Authors FREITAS, Vanessa C, PARREIRAS, Klívia P, DUARTE, Ana Paula M, SECUNDINO, Nágila F. C, PIMENTA, Paulo F. P
Format Journal Article
LanguageEnglish
Published Deerfield, IL American Society of Tropical Medecine and Hygiene 01.04.2012
The American Society of Tropical Medicine and Hygiene
Subjects
Animals
Biological and medical sciences
Female
Infectious diseases
Insect Vectors - parasitology
Leishmania infantum
Leishmania infantum - growth & development
Leishmania infantum - isolation & purification
Leishmaniasis, Visceral - transmission
Life Cycle Stages
Lutzomyia longipalpis
Medical sciences
Protozoan Proteins - isolation & purification
Psychodidae - parasitology
Reverse Transcriptase Polymerase Chain Reaction - methods
Kinetoplastida
Protozoa
Leishmania chagasi
Leishmania infantum
Phlebotomus
Arthropoda
Insecta
Psychodidae
Tropical medicine
Invertebrata
Vector
Diptera
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Summary:We analyzed the development of Leishmania (Leishmania) infantum chagasi in its natural sandfly vector Lutzomyia longipalpis. In addition, we compared sandfly infections initiated with axenic amastigotes or promastigotes. Our data showed no important difference between Lu. longipalpis infection rates resulting from either type of infections. Furthermore, development of infection was equivalent in both cases. All promastigote forms were found inside the sandfly and, after blood digestion, most of the population consisted of procyclics and nectomonads. A low percentage of metacyclic forms was coincident with a high number of nectomonads during late stages of infection, but which form gives rise to metacyclic forms in L. infantum chagasi is unknown. These results also show that the promastigote infection model, at least for this situation, is suitable for obtaining of infected sandflies because it is easier and less laborious.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2012.11-0386