A highly selective and sensitive fluorescent probe for hypochlorous acid and its lysosome-targetable biological applications

• Published in: Talanta (Oxford) Vol. 174; pp. 234 - 242
• Main Authors: Liu, Chang, Jiao, Xiaojie, He, Song, Zhao, Liancheng, Zeng, Xianshun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017
• Subjects: 1,8-Naphthalimide; electron transfer; fluorescence; fluorescent dyes; Fluorescent probe; Hypochlorous acid; image analysis; Imaging; Lysosome; monitoring; morpholine; PET; reactive oxygen species; Hypochlorous acid; 1,8-Naphthalimide; Imaging; PET; Fluorescent probe; Lysosome
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2017.06.012

A novel lysosome-targetable fluorescence probe PT-1 based on a photoinduced electron transfer (PET) mechanism has been designed and synthesized. For comparison, the probe PT-2 without morpholine moiety as a lysosome-directing group was also synthesized and investigated. Probes PT-1 and PT-2 exhibited high selectivity, high sensitivity (with the detection limits down to the 10−10M range) and response in real time (within 10s) toward HOCl over other reactive oxygen species (ROS). Both PT-1 and PT-2 were cell permeable and enabled them to be used for monitoring of HOCl in living cells. Meanwhile, the probe PT-1 demonstrated an accurately lysosome-targeting ability, and was successfully applied to image of exogenous, endogenous produced HOCl in living cells. The success of subcellular imaging suggested that the probe PT-1 could be used in further applications for the investigation of biological functions and pathological roles of HOCl at organelle levels. A novel lysosome-targetable fluorescence probe has been rationally designed and synthesized for sensitive and selective detection of HOCl. The probe functions as an excellent fluorescence probe for real-time imaging of HOCl in lysosomes. [Display omitted] •A novel lysosome-targetable fluorescence probe has been rationally designed and synthesized.•The probe exhibits high selectivity and sensitivity for the detection of HOCl.•The probe functions as an excellent fluorescence probe for real-time imaging of HOCl in lysosomes.