Efficacy of methotrexate-loaded poly( -caprolactone) implants in Ehrlich solid tumor-bearing mice

• Published in: Drug delivery Vol. 20; no. 3-4; pp. 168 - 179
• Main Authors: Pereira, Adriana De Fátima, Pereira, Leticia Gonçalves Resende, Barbosa, Leandro Augusto De Oliveira, Fialho, Sílvia Ligório, Pereira, Bruno Gonçalves, Patricio, Patrícia Santiago De Oliveira, Pinto, Flávia Carmo Horta, Da Silva, Gisele Rodrigues
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare USA, Inc 01.04.2013; Taylor & Francis
• Subjects: Animals; Antitumor activities; Carcinoma, Ehrlich Tumor - drug therapy; Carcinoma, Ehrlich Tumor - pathology; Drug Implants; Ehrlich solid tumor; Female; HeLa Cells; Humans; implantable drug delivery system; implants; methotrexate; Methotrexate - administration & dosage; Methotrexate - chemistry; Mice; poly(ε-caprolactone); Polyesters - administration & dosage; Polyesters - chemistry; Treatment Outcome; X-Ray Diffraction
• ISSN: 1071-7544; 1521-0464
• DOI: 10.3109/10717544.2013.801052

Abstract Context: Methotrexate (MTX) is used in the treatment of malignancies; however, its clinical application is limited by its toxic dose-related side effects. An alternative to overcome the toxicity of the MTX in healthy tissues is the design of an implantable device capable of controlling the delivery of this drug for an extended period within the tumor site. Objective: To develop methotrexate-loaded poly( -caprolactone) implants (MTX PCL implants) and to demonstrate their efficacy as local drug delivery systems capable of inhibiting Ehrlich solid tumor bearing mice. Materials and methods: MTX PCL implants were produced by the melt-molding technique and were characterized by FTIR, WAXS, DSC and SEM. The in vitro and in vivo release of MTX from the PCL implants was also evaluated. The efficacy of implants in inhibiting tumor cells in culture and the solid tumor in a murine model was revealed. Results and discussion: The chemical and morphological integrity of the drug was preserved into the polymeric matrix. The in vitro and in vivo release processes of the MTX from the PCL implants were modulated by diffusion. MTX diffused from the implants revealed an antiproliferative effect on tumor cells. Finally, MTX controlled and sustained released from the polymeric implants efficiently reduced 42.7% of the solid tumor in mice paw. Conclusion: These implantable devices represented a contribution to improve the efficacy and safety of chemotherapy treatments, promoting long-term local drug accumulation in the targeted site.