The Use of a Prostacyclin Analog, Iloprost, as an Adjunct to Uterus Preservation with Histidine-Tryptophan-Ketoglutarate Solution

• Published in: Transplantation proceedings Vol. 43; no. 5; pp. 1998 - 2003
• Main Authors: Barun, S, Özat, M, Güngor, T, Demiroǧullari, B, Sökmensüer, L.K, Aksakal, F.N, Erçin, U, Gülbahai, Ö, Müftüoğlu, S
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.06.2011; Elsevier
• Subjects: Animals; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glucose; Iloprost - pharmacology; Mannitol; Medical sciences; Potassium Chloride; Preservation, Biological; Procaine; Rats; Rats, Wistar; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue, organ and graft immunology; Uterus - drug effects; Iloprost; Vasodilator agent; Prostaglandin I2; Preservation; Arachidonic acid derivatives; Use; Tryptophan; Transplantation; Antiplatelet agent; Medicine; Organ preservation; Histidine; Treatment; Uterus; Aminoacid; Surgery; Analog; Female genital system; Antithrombotic agent; Graft; Solution; Prostaglandin derivatives
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2011.04.005

Abstract Introduction Although assisted reproductive techniques have made most causes of both male and female infertility treatable, uterine factor infertility is not able to therapy. Therefore, transplantation of the uterus has been suggested as a future possible cure. Organ preservation solutions seek to reduce reperfusion injury. Since iloprost is an antioxidant with cytoprotective properties, we investigated its potential positive effects in histidine-tryptophan-ketoglutarate (HTK) solution after 4 or 24 h cold storage period of the rat uterus. Methods We divided 24 female Wistar-albino rats into four groups: Group 1 had the uterus tissue stored in HTK solution at 4 °C for 4h. Group 2, the tissue was stored in HTK solution combined with iloprost (10−8 M) for 4h at 4 °C. The same procedures were repeated for 24 h for Groups 3 and 4 respectively. Tissue levels of malondialdehyde (MDA) and nitric oxide (NO), as indicators of oxidative stress were determined with histopathological evaluations. Results MDA and NO levels were compared between the group 1 vs 3; and 2 vs 4. No significant difference was observed between the groups. Cold storage for 24 h produced alterations in histological appearences that were mitigated by the addition of iloprost to HTK solution. Conclusion In conclusion, addition of iloprost to HTK solution reversed the histological alterations after 24h-cold storage of the rat uterus.