Acinetobacter baumannii Multidrug-Resistant Strain Occurrence in Liver Recipients With Reference to Other High-Risk Groups

• Published in: Transplantation proceedings Vol. 43; no. 8; pp. 3116 - 3120
• Main Authors: Netsvyetayeva, I, Sikora, M, Golas, M, Swoboda-Kopec, E, Walter de Walthoffen, S, Dembicka, O, Fraczek, M, Mlynarczyk, A, Pacholczyk, M, Chmura, A, Mlynarczyk, G
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier Inc 01.10.2011; Elsevier
• Subjects: Acinetobacter baumannii - drug effects; Acinetobacter baumannii - enzymology; Acinetobacter baumannii - genetics; Acinetobacter Infections - drug therapy; Acinetobacter Infections - etiology; Acinetobacter Infections - microbiology; Bacterial Proteins - genetics; Base Sequence; beta-Lactamases - genetics; Biological and medical sciences; DNA, Bacterial - genetics; Drug Resistance, Multiple, Bacterial - genetics; Fractures, Bone - surgery; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genes, Bacterial; Humans; Liver Transplantation - adverse effects; Medical sciences; Neoplasms - complications; Postoperative Complications - drug therapy; Postoperative Complications - etiology; Postoperative Complications - microbiology; Risk Factors; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical Wound Infection - drug therapy; Surgical Wound Infection - microbiology; Tissue, organ and graft immunology; Human; High risk; Treatment resistance; Digestive system; Liver; Reference; Neisseriaceae; Recipient; Transplantation; Strain; Medicine; Acinetobacter baumannii; Treatment; Surgery; Multiple resistance; Bacteria; Micrococcales; Graft
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2011.07.008

Abstract Introduction The increasing clinical significance of Acinetobacter baumannii species is due to its ability to survive in hospital environments, its species-specific multidrug resistance, and its ability to instantly develop various drug-resistance mechanisms through antibiotic pressure. Materials and methods We identified 16 A baumannii strains isolated from patients presenting postoperative infections in 2010. A baumannii isolates were obtained from clinical specimens by standard microbiologic methods. As previously described, we performed polymerase chain reaction (PCR) analysis for carbapenemase-encoding genes (VIM, IMP, SPM, OXA23, OXA24, OXA51, OXA58) in Acinetobacter spp. Results The double-disk synergy test phenotypic method did not detect any A baumannii strains producing metallo-beta-lactamaus cultured from swabs from all the patient groups. No products of PCR amplification with specific starters for VIM, IMP, and SPM (Sao Paulo metallo-β-lactamase) genes were found. All analyzed strains were colistin-sensitive. Among five strains from liver recipients, one was imipenem- and meropenem-resistant. Four among six strains isolated from cancer patients were resistant to imipenem and/or meropenem; 1/5 were imipenem-and meropenem-resistant; 1, meropenem-resistant and imipenem-sensitive; 1, meropenem- and imipenem-resistant; and 1 with intermediate resistance to both meropenem and imipenem among swabs cultured from patients with postoperative complication after bone fracture. Fifteen among 16 analyzed A baumannii strains had an OXA51 gene. Two among five A baumannii strains isolated in liver recipients had only an OXA51 gene; one, OXA51 and OXA24 genes; one, OXA51 and OXA23 genes.