Human malic enzymes in heart and muscle: Evidence of a selective distribution

• Published in: Biochemical medicine and metabolic biology Vol. 45; no. 1; pp. 1 - 5
• Main Authors: Tessarolo, D., Liguori, M., Giacanelli, M.
• Format: Journal Article
• Language: English
• Published: Boston, MA Elsevier Inc 01.02.1991; San Diego, CA Academic Press; New York, NY
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Electrophoresis, Starch Gel; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; heart; Humans; malate dehydrogenase (decarboxylase); Malate Dehydrogenase - metabolism; man; Muscles - enzymology; Myocardium - enzymology; Oxidoreductases; purification; skeletal muscle; Tissue Distribution; Heart; Human; Mitochondria; Enzyme; Isozyme; Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); Tissue specificity; Malate dehydrogenase(decarboxylating); Striated muscle
• ISSN: 0885-4505; 1557-7651
• DOI: 10.1016/0885-4505(91)90002-3

The NADP +-dependent activity of malic enzymes EC 1.1.1.39 and EC 1.1.1.40 was studied in human cardiac and skeletal muscle obtained from living subjects. We used polyacrylamide gel electrophoresis to detect and extract the enzymatic forms and starch gel electrophoresis to confirm their identification. This simple procedure allowed us to provide evidence of a selective NADP +-dependent distribution of malic enzyme activities between the two muscular tissues, using a smaller amount of sample than used with previous methods.