Glucose‐regulated protein 75 suppresses apoptosis induced by glucose deprivation in PC12 cells through inhibition of Bax conformational change

Glucose‐regulated protein 75 (Grp75) is an important molecular chaperone that belongs to the heat shock protein 70 family and resides predominantly in mitochondria. Grp75 can protect cells from glucose deprivation (GD) injury. However, the molecular mechanisms by which it carries out this function a...

Full description

Saved in:
Bibliographic Details
Published inActa biochimica et biophysica Sinica Vol. 40; no. 4; pp. 339 - 348
Main Authors Yang, Ling, Liu, Xiaoyu, Hao, Jinyu, Yang, Yunlong, Zhao, Mingxia, Zuo, Ji, Liu, Wen
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.04.2008
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Glucose‐regulated protein 75 (Grp75) is an important molecular chaperone that belongs to the heat shock protein 70 family and resides predominantly in mitochondria. Grp75 can protect cells from glucose deprivation (GD) injury. However, the molecular mechanisms by which it carries out this function are unknown. Here we report that Grp75 could delay the release of cytochrome c and reduce apoptosis induced by GD, and we also found that Grp75 could decrease Bax/Bcl‐2 gene expression ratio and inhibit the conformational change of Bax during this process. In conclusion, these findings suggested that Grp75 overexpression was able to inhibit apoptosis induced by GD. Grp75 inhibited Bax conformational change to delay the release of cytochrome c, thus providing protection to PC12 cell which was used primarily as a neuron model against GD toxicity.
Bibliography:This work was supported by a grant from the Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20050246079)
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1672-9145
1745-7270
DOI:10.1111/j.1745-7270.2008.00409.x