Improving detection of Vibrio vulnificus in Octopus variabilis by PCR

• Published in: Journal of food science Vol. 62; no. 1; pp. 179 - 182
• Main Authors: Lee, J.Y. (Chonnam National Univ., Korea.), Eun, J.B, Choi, S.H
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1997; Institute of Food Technologists; Wiley Subscription Services, Inc
• Subjects: AGENT PATHOGENE; Bacteria; Biological and medical sciences; Deoxyribonucleic acid; DNA; DNA extraction; ENFERMEDADES TRANSM POR ALIMENTOS; Fish and seafood industries; Food contamination & poisoning; Food industries; FOODBORNE DISEASES; Fundamental and applied biological sciences. Psychology; MALADIE TRANSMISSIBLE PAR ALIMENT; Marine; OCTOPUS; OCTOPUS (GENUS); Octopus variabilis; ORGANISMOS PATOGENOS; PATHOGENS; PCR; rapid enrichment; Seafood; small octopus; VIBRIO VULNIFICUS; Enrichment; Edible mollusc; Microbiological testing; Vibrio vulnificus; Octopus; Optimization; Polymerase chain reaction; Cephalopoda; Rapid technique; Pathogenic; Microorganism culture; Bacteria; Vibrionaceae; Invertebrata; Mollusca; Detection; Biological contamination
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/j.1365-2621.1997.tb04395.x

PCR methods can detect foodborne pathogenic bacteria with simplicity, specificity and speed. In order to improve sensitivity and speed of PCR methods for detection of Vibrio vulnificus in small octopus homogenate, several media and culture conditions were compared. Modified brain heart infusion media containing 2% NaCl and adjusted to pH 8.0 and 30degrees C was most effective for enrichment of the bacteria. Procedures affecting the efficiency of template DNA extraction and target DNA amplification were also optimized. By these combined efforts, a PCR procedure capable of detecting V. vulnificus as low as 10 cells/mL within 10h was developed