Characterization of hair lipid images by argon sputter etching-scanning electron microscopy

• Published in: Lipids Vol. 41; no. 2; pp. 197 - 205
• Main Authors: Masukawa, Yoshinori, Tanamachi, Hiroto, Tsujimura, Hisashi, Mamada, Akira, Imokawa, Genji
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.02.2006; Springer‐Verlag; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; Argon; Asian Continental Ancestry Group; Cell Membrane - chemistry; Child; Child, Preschool; European Continental Ancestry Group; Female; Fibers; Hair; Hair - chemistry; Hair - ultrastructure; Humans; Lipids; Lipids - chemistry; Microscopy, Electron, Scanning - methods; Middle Aged; Physicochemical properties
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-006-5088-4

Hair lipid images, as visualized by argon sputter etching-scanning electron microscopy (ASE-SEM), reveal convex structures with a stitch pattern (SP) at the cell membrane complex (CMC) in the transverse hair plane. Based on interindividual variation, different features of the convex SP were classified into Types 0 to 4 with the corresponding scores 0 to 4. Observations using hair fibers collected from 27 Japanese females revealed significant positive correlations between the scores and the levels of exogenous lipids, which suggests that exogenous lipids internalized at the CMC predominantly constitute the convex SP. Intraindividual variation with different levels of exogenous lipids among hair fibers derived from individual females may be relevant to the uneven physicochemical properties of hair fibers on the scalp. Observations of 380 hair fibers collected from Japanese (Mongoloid), German and American (Caucasoid) females aged 3 to 77 yr demonstrated similar age-related changes in the lipid images, which represent an increase and then a decrease in levels of exogenous lipids with increasing age. This suggests that age-related changes in exogenous lipids are attributable to alterations in sebum excreted during aging and that this elicits age-related changes in physical parameters, which affect human hair texture.