Relative Orientation of Rigid Nitroxides by PELDOR: Beyond Distance Measurements in Nucleic Acids

• Published in: Angewandte Chemie (International ed.) Vol. 48; no. 18; pp. 3292 - 3295
• Main Authors: Schiemann, Olav, Cekan, Pavol, Margraf, Dominik, Prisner, Thomas F, Sigurdsson, Snorri Th
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 20.04.2009; WILEY‐VCH Verlag; Wiley
• Subjects: Chemistry; Chemistry, Multidisciplinary; DEER spectroscopy; DNA - chemistry; Electron Spin Resonance Spectroscopy - methods; EPR spectroscopy; Nitrous Oxide - chemistry; Nucleic Acid Conformation; nucleic acids; orientations; Oxides - chemistry; Physical Sciences; Science & Technology; site‐directed spin labeling; Spin Labels; NUCLEOSIDE; DEER spectroscopy; site-directed spin labeling; SPIN; SPECTROSCOPY; BIOMACROMOLECULES; orientations; ELECTRON DOUBLE-RESONANCE; nucleic acids; PROBE; EPR spectroscopy; PULSED ELECTRON

Show me your angle: Incorporation of the rigid spin label Ç allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X-band frequencies. The orientational information is obtained by varying the position of the detection pulses over the...