Relative Orientation of Rigid Nitroxides by PELDOR: Beyond Distance Measurements in Nucleic Acids

Show me your angle: Incorporation of the rigid spin label Ç allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X-band frequencies. The orientational information is obtained by varying the position of the detection pulses over the...

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Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 48; no. 18; pp. 3292 - 3295
Main Authors Schiemann, Olav, Cekan, Pavol, Margraf, Dominik, Prisner, Thomas F, Sigurdsson, Snorri Th
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 20.04.2009
WILEY‐VCH Verlag
Wiley
Subjects
Chemistry
Chemistry, Multidisciplinary
DEER spectroscopy
DNA - chemistry
Electron Spin Resonance Spectroscopy - methods
EPR spectroscopy
Nitrous Oxide - chemistry
Nucleic Acid Conformation
nucleic acids
orientations
Oxides - chemistry
Physical Sciences
Science & Technology
site‐directed spin labeling
Spin Labels
NUCLEOSIDE
DEER spectroscopy
site-directed spin labeling
SPIN
SPECTROSCOPY
BIOMACROMOLECULES
orientations
ELECTRON DOUBLE-RESONANCE
nucleic acids
PROBE
EPR spectroscopy
PULSED ELECTRON
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Summary:Show me your angle: Incorporation of the rigid spin label Ç allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X-band frequencies. The orientational information is obtained by varying the position of the detection pulses over the nitroxide spectrum. Simulation of the set of time traces yields very precise distances and angles.
Bibliography:http://dx.doi.org/10.1002/anie.200805152
This work was supported by the Icelandic Research Fund (60028021), the DFG (SFB 579, RNA Ligand Interaction), the Center of Biomolecular Magnetic Resonance, the BBSRC, an RCUK fellowship to O.S. and a doctoral fellowship to P.C. from the Eimskip Fund of the University of Iceland.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200805152