Quantifying the Performance of Protein-Resisting Surfaces at Ultra-Low Protein Coverages using Kinesin Motor Proteins as Probes

The binding kinetics of fluorescently labeled microtubules to kinesin‐coated surfaces permits the determination of the density of kinesin motor proteins adhered to the surface in the range of 0.1 – 30 μm–2. This extreme sensitivity, corresponding to protein coverages of 0.004 – 1 ng cm–2, enables th...

Full description

Saved in:
Bibliographic Details
Published inAdvanced materials (Weinheim) Vol. 19; no. 20; pp. 3171 - 3176
Main Authors Katira, P., Agarwal, A., Fischer, T., Chen, H.-Y., Jiang, X., Lahann, J., Hess, H.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 19.10.2007
WILEY‐VCH Verlag
Subjects
Biomedical materials
Bionanotechnology
Coatings
Microtubes
Proteins
Online AccessGet full text

Cover

More Information
Summary:The binding kinetics of fluorescently labeled microtubules to kinesin‐coated surfaces permits the determination of the density of kinesin motor proteins adhered to the surface in the range of 0.1 – 30 μm–2. This extreme sensitivity, corresponding to protein coverages of 0.004 – 1 ng cm–2, enables the characterization of advanced non‐fouling coatings, such as (EG)3OH‐terminated SAMs and PEGMA with applications in biomedical engineering and bionanotechnology.
Bibliography:Alexander-von-Humboldt foundation
P.K. and A.A. contributed equally to this work. H.H. gratefully acknowledges financial support from the DOE Office of Basic Energy Sciences, the DARPA-DSO Biomolecular Motors Program (FA9550-05-1-366). T.F. thanks the Alexander-von-Humboldt foundation for support through a Feodor Lynen fellowship. J.L. gratefully acknowledges support from the NSF in form of a CAREER grant (DMR-0449462) and funding form the NSF under the MRI program (DMR 0420785).
DARPA-DSO Biomolecular Motors Program - No. FA9550-05-1-366
ark:/67375/WNG-3P74TQWG-N
NSF - No. DMR-0449462; No. DMR 0420785
ArticleID:ADMA200701982
DOE Office of Basic Energy Sciences
istex:7ACD5FF983750FCD95F8021BACA3E0F92A1A1D25
P.K. and A.A. contributed equally to this work. H.H. gratefully acknowledges financial support from the DOE Office of Basic Energy Sciences, the DARPA‐DSO Biomolecular Motors Program (FA9550‐05‐1‐366). T.F. thanks the Alexander‐von‐Humboldt foundation for support through a Feodor Lynen fellowship. J.L. gratefully acknowledges support from the NSF in form of a CAREER grant (DMR‐0449462) and funding form the NSF under the MRI program (DMR 0420785).
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0935-9648
1521-4095
DOI:10.1002/adma.200701982