A study of biologically active peptide sequences (P-15) on the surface of an ABM scaffold (PepGen P-15™) using AFM and FTIR
Cellular response to any biomaterial surface is governed by a number of factors including topography, surface chemistry, surface charge, structural heterogeneity, and physiological conditions. Understanding these factors at the nanoscale level is crucial to develop improved biomaterials. Any changes...
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Published in | Journal of biomedical materials research. Part A Vol. 74A; no. 4; pp. 712 - 721 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
15.09.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Cellular response to any biomaterial surface is governed by a number of factors including topography, surface chemistry, surface charge, structural heterogeneity, and physiological conditions. Understanding these factors at the nanoscale level is crucial to develop improved biomaterials. Any changes in these properties due to surface modifications need to be addressed properly, as they could have significant impact on the cellular interaction with biomaterials. In this study, the topography and surface chemistry of commercially available tissue engineered xenograft, PepGen P‐15™ [comprised of a synthetic peptide P‐15 irreversibly attached to anorganic bovine bone mineral (OsteoGraf/‐N®)] was studied using Atomic Force Microscopy (AFM), and Fourier Transform Infrared Spectroscopy (FTIR). FTIR confirmed the presence of the peptide on the surface of PepGen P‐15™. Changes in the peptide conformation, which includes a decrease in the β‐strand accompanied by an increase in unordered structures/random coil structures after attachment on OsteoGraf/‐N® is observed. Specific functional groups, which are involved in the binding mechanism, are identified. The results suggest that the attachment of the peptide on OsteoGraf/‐N® occurs via a specific surface docking ionic interaction involving the C‐terminal carboxylic group on the peptide with positive domains generated by hydroxyl vacancies on the apatite surface. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005 |
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Bibliography: | ark:/67375/WNG-6FNRDHMB-7 ArticleID:JBM30331 istex:D23B69DAA1276594149C4BEE6F2973EFC220A196 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1549-3296 1552-4965 |
DOI: | 10.1002/jbm.a.30331 |