Improvement of cell adhesion on poly(L-lactide) by atmospheric plasma treatment

• Published in: Journal of biomedical materials research. Part A Vol. 77A; no. 1; pp. 112 - 118
• Main Authors: Nakagawa, Masafumi, Teraoka, Fumio, Fujimoto, Shinji, Hamada, Yoshinosuke, Kibayashi, Hiroyuki, Takahashi, Junzo
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2006
• Subjects: 3T3 Cells; Animals; Atmosphere; atmospheric plasma treatment; Cell Adhesion; Electrochemistry; MC3T3-E1 cell; Mice; Poly(L-lactide) (PLLA); Polyesters; Surface Properties; tissue engineering; Water
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.30521

The purpose of this study is to elucidate the interaction between the cell and the surface of poly(L‐lactide) (PLLA) samples, which were modified using a low‐temperature plasma treatment apparatus at atmospheric pressure. The plasma treatments were carried out in the atmospheres of air, carbon dioxide (CO2), and perfluoro propane (C3F8) gas. The PLLA samples before and after the plasma treatment were analyzed by XPS and their contact angles with water. Furthermore, the cell adhesion capability and cell mass culturing tests on the PLLA samples were carried out using MC3T3‐E1 cells. The results showed that the contact angle of the samples, which was plasma treated in air or in CO2 gas, decreased compared with that of the untreated samples. On the other hand, the contact angle of the samples, which was plasma treated in the C3F8 gas, increased compared with the untreated plasma samples. The cell response on the PLLA samples plasma treated in air or in the CO2 gas were significantly superior to that of the PLLA samples, which was plasma treated in the C3F8 gas. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006