A High-Affinity Binding Protein for the Regulatory Subunit of cAMP-Dependent Protein Kinase II in the Centrosome of Human Cells

In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RIIα and RIIβ of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RIIα and RIIβ show that t...

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Published inExperimental cell research Vol. 204; no. 2; pp. 230 - 240
Main Authors Keryer, Guy, Rios, Rosa Maria, Landmark, Brynjar F., Skalhegg, Bjorn, Lohmann, Suzanne M., Bornens, Michel
Format Journal Article
LanguageEnglish
Published Orlando, FL Elsevier Inc 01.02.1993
Elsevier
Subjects
Biological and medical sciences
Cell Line
Cell nucleus
Cell structures and functions
Centrioles - chemistry
Centrioles - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Molecular and cellular biology
Protein Binding
Protein Kinases - metabolism
Proteins - analysis
Human
Binding protein
Molecular form
Regulation(control)
Cell line
Enzyme
Protein kinase
Cyclic AMP
Localization
Centrosome
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Summary:In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RIIα and RIIβ of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RIIα and RIIβ show that these two isoforms are expressed. The major isoform α is mostly cytosolic, whereas the β isoform appears concentrated in the Golgi-centrosomal area, as judged by immunofluorescence and cell fractionation. Using a 32P-labeled RII overlay on Western blots, a 350-kDa RII-binding protein (AKAP 350) was specifically identified in centrosomes isolated from this cell line, whereas a Golgi fraction has previously been demonstrated to contain an 85-kDa RII-binding protein (AKAP 85). AKAP 350 is highly insoluble and can partially be extracted from centrosomes as a complex of AKAP 350 and RII subunit. AKAP 350 was identified as a specific centrosomal protein previously demonstrated in the pericentriolar material. The potential significance of a specific subcellular distribution for different RII-binding proteins in nonneuronal cells is discussed.
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content type line 23
ISSN:0014-4827
1090-2422
DOI:10.1006/excr.1993.1029