Lipase-catalysed transesterification: Viewpoint of the mechanism and influence of free fatty acids

• Published in: Biomass & bioenergy Vol. 85; pp. 94 - 99
• Main Authors: Canet, Albert, Bonet-Ragel, Kírian, Benaiges, M. Dolors, Valero, Francisco
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2016
• Subjects: alcoholysis; biocatalysts; Biodiesel; diacylglycerols; esterification; Free fatty acids; hydrolysis; linoleic acid; Lipase; Lipase inactivation; Rhizopus oryzae; transesterification; Transesterification mechanism; triolein; Transesterification mechanism; Rhizopus oryzae; Lipase inactivation; Lipase; Biodiesel; Free fatty acids
• ISSN: 0961-9534; 1873-2909
• DOI: 10.1016/j.biombioe.2015.11.021

A series of lipase-catalysed transesterification experiments were carried out to study the effect of the presence of free fatty acids on synthesis reaction rate and the stability of the biocatalyst, and also to elucidate the underlying process, which remains a subject of debate. Based on the results, the reaction rate and biocatalyst stability increased with increasing content in free fatty acids of the reaction mixture. Also, tests carried out with a mixture of triolein and linoleic acid revealed that the transesterification is a combination of direct alcoholysis of triacylglycerols and a two-step reaction involving hydrolysis of acylglycerols and further esterification of previously released free fatty acids. The time course of triacylglycerols and diacylglycerols revealed that the enzyme is similarly selective for both types of substrate. •Free fatty acids increase lipase stability.•Elucidated mechanisms transesterification.•Free fatty acids increase biodiesel reaction rate.