Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells

• Published in: Cellular physiology and biochemistry Vol. 49; no. 1; pp. 101 - 122
• Main Authors: Chen, Xuxin, Zhang, Yinliang, Wang, Wenjing, Liu, Zhenqian, Meng, Jiguang , Han, Zhihai
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2018; Cell Physiol Biochem Press GmbH & Co KG
• Subjects: Acute lung injury (ALI); Animals; Antibiotics; Bone marrow; Cell Differentiation; Cell Survival; Cytokines; Cytokines - analysis; Cytokines - metabolism; Edema; Endothelial Cells - cytology; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Endothelium; Experiments; Heart attacks; Heme Oxygenase-1 (HO-1); Heme Oxygenase-1 - antagonists & inhibitors; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; Humans; Hyperoxia; Hypoxia; Inflammation; Inflammation - prevention & control; Ischemia; Laboratory animals; Lipopolysaccharide (LPS); Lipopolysaccharides - toxicity; Mesenchymal Stem Cells (MSCs); Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; NF-E2-Related Factor 2 - metabolism; NF-kappa B - metabolism; Original Paper; Oxidative Stress; Oxidative Stress - drug effects; Paracrine; Paracrine Communication; Penicillin; Proteins; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Respiratory distress syndrome; RNA Interference; RNA, Small Interfering - metabolism; Rodents; Stem cells; Superoxide Dismutase - metabolism; United States > US; Oxidative Stress; Paracrine; Heme Oxygenase-1 (HO-1); Inflammation; Mesenchymal Stem Cells (MSCs); Acute lung injury (ALI); Lipopolysaccharide (LPS)
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000492847

Background/Aims: Bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation has therapeutic effects on endothelial damage during acute lung injury (ALI). Heme oxygenase-1 (HO-1) can restore homeostasis and implement cytoprotective defense functions in many pathologic states. Therefore, we explored whether transduction of HO-1 into BM-MSCs (MSCs-HO-1) would have an increased beneficial effect on lipopolysaccharide (LPS)-induced inflammatory and oxidative damage in human pulmonary microvascular endothelial cells (PVECs). Methods: MSCs were isolated from rat bone marrow and transfected with the HO-1 gene by a lentivirus vector. The phenotype and multilineage differentiation of MSCs were assessed. MSCs or MSCs-HO-1 were co-cultured with PVECs using a transwell system, and LPS was added to induce PVEC injury. The production of reactive oxygen species (ROS), and the activities of lipid peroxide (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in PVECs were determined by flow cytometry and colorimetric assays, respectively. The levels of human PVEC-derived tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in the supernatants of the co-culture system, and the activity of nuclear transcription factor-κB and NF-E2-related factor 2 (Nrf2) in PVECs were examined by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of TNF-α, IL-1β and IL-6 in PVECs was detected by quantitative real-time polymerase chain reaction (qRT-PCR), HO-1 expression and enzymatic activity in PVECs and the influence of zinc protoporphyrin (ZnPP) or HO-1 small interfering RNA on the above inflammatory and oxidative stress markers were evaluated. In addition, the expression of rat MSC-derived hepatocyte growth factor (HGF) and IL-10 was determined by ELISA and qRT-PCR. Results: MSCs showed no significant changes in phenotype or multilineage differentiation after transduction. LPS strongly increased the production of inflammatory and oxidative stress indicators, as well as decreased the levels of antioxidant components and the activity of Nrf2 in PVECs. MSC co-cultivation ameliorated these detrimental effects in PVECs and MSCs-HO-1 further improved the damage to PVECs induced by LPS when compared with MSCs alone. The beneficial effects of MSCs-HO-1 were dependent on HO-1 overexpression and may be attributed to the enhanced paracrine production of HGF and IL-10. Conclusion: MSCs-HO-1 have an enhanced ability to improve LPS-induced inflammatory and oxidative damage in PVECs, and the mechanism may be partially associated with the enhanced paracrine function of the stem cells. These data encourage further testing of the beneficial effects of MSCs-HO-1 in ALI animal models.